Purpose: Pulsatile delivery of proteins, in which release occurs over a short time after a period of little or no release, is desirable for many applications. This paper investigates the effect of biodegradable polymer shell thickness on pulsatile protein release from biodegradable polymer microcapsules.
Methods: Using precision particle fabrication (PPF) technology, monodisperse microcapsules were fabricated encapsulating bovine serum albumin (BSA) in a liquid core surrounded by a drug-free poly(lactide-co-glycolide) (PLG) shell of uniform, controlled thickness from 14 to 19 μm.
Results: When using high molecular weight PLG (Mw 88 kDa), microparticles exhibited the desired core-shell structure with high BSA loading and encapsulation efficiency (55-65%). These particles exhibited very slow release of BSA for several weeks followed by rapid release of 80-90% of the encapsulated BSA within 7 days. Importantly, with increasing shell thickness the starting time of the pulsatile release could be controlled from 25 to 35 days.
Conclusions: Biodegradable polymer microcapsules with precisely controlled shell thickness provide pulsatile release with enhanced control of release profiles.
|Number of pages||10|
|State||Published - Nov 2014|
Bibliographical noteFunding Information:
This work was supported by the National Institute of Health Grant EB005181 and GM085222. Scanning Electron Microscopy took place in Material Research Lab at University of Illinois at Urbana-Champaign. Confocal microscopy measurements took place in Beckman Institute, imaging technology group at University of Illinois at Urbana-Champaign. DSC was performed in Microanalysis Laboratory, School of Chemical Sciences, University of Illinois at Urbana-Champaign.
© 2014 Springer Science+Business Media New York.
- bovine serum albumin
- controlled release
- monodisperse microcapsules
- pulsatile release
ASJC Scopus subject areas
- Molecular Medicine
- Pharmaceutical Science
- Organic Chemistry
- Pharmacology (medical)