Abstract
Purpose: Pulsatile delivery of proteins, in which release occurs over a short time after a period of little or no release, is desirable for many applications. This paper investigates the effect of biodegradable polymer shell thickness on pulsatile protein release from biodegradable polymer microcapsules.
Methods: Using precision particle fabrication (PPF) technology, monodisperse microcapsules were fabricated encapsulating bovine serum albumin (BSA) in a liquid core surrounded by a drug-free poly(lactide-co-glycolide) (PLG) shell of uniform, controlled thickness from 14 to 19 μm.
Results: When using high molecular weight PLG (Mw 88 kDa), microparticles exhibited the desired core-shell structure with high BSA loading and encapsulation efficiency (55-65%). These particles exhibited very slow release of BSA for several weeks followed by rapid release of 80-90% of the encapsulated BSA within 7 days. Importantly, with increasing shell thickness the starting time of the pulsatile release could be controlled from 25 to 35 days.
Conclusions: Biodegradable polymer microcapsules with precisely controlled shell thickness provide pulsatile release with enhanced control of release profiles.
| Original language | English |
|---|---|
| Pages (from-to) | 3201-3210 |
| Number of pages | 10 |
| Journal | Pharmaceutical Research |
| Volume | 31 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 2014 |
Bibliographical note
Publisher Copyright:© 2014 Springer Science+Business Media New York.
Funding
This work was supported by the National Institute of Health Grant EB005181 and GM085222. Scanning Electron Microscopy took place in Material Research Lab at University of Illinois at Urbana-Champaign. Confocal microscopy measurements took place in Beckman Institute, imaging technology group at University of Illinois at Urbana-Champaign. DSC was performed in Microanalysis Laboratory, School of Chemical Sciences, University of Illinois at Urbana-Champaign.
| Funders | Funder number |
|---|---|
| National Institutes of Health (NIH) | R01GM085222 |
| National Institutes of Health (NIH) | |
| National Institute of Biomedical Imaging and Bioengineering | R01EB005181 |
| National Institute of Biomedical Imaging and Bioengineering |
Keywords
- bovine serum albumin
- controlled release
- monodisperse microcapsules
- poly(lactide-co-glycolide)
- pulsatile release
ASJC Scopus subject areas
- Biotechnology
- Molecular Medicine
- Pharmacology
- Pharmaceutical Science
- Organic Chemistry
- Pharmacology (medical)