TY - JOUR
T1 - Purification and Characterization of Lipase from Rhizomucor miehei
AU - Uvarani, Gangaraju
AU - Jaganathan, Lakshmanan
AU - Shridas, Preetha
AU - Boopathy, Rathanam
PY - 1998
Y1 - 1998
N2 - The production of Rhizomucor miehei lipase by solid state fermentation by growing the fungi for 5 days at room temperature on wheat bran medium with a moisture content of 0.75 mL/g is described. The purification of this extracellular enzyme to apparent homogeneity employing a two-step purification sequence using ion-exchange and Octyl-Sepharose affinity chromatography with 135-fold purity and 12% recovery is reported. The purified extracellular enzyme displays maximum activity at pH 7.0 and 65°C. The characteristic feature of this enzyme is its thermostability, retaining 90% of its activity upon exposure to 65°C for 30 min. Further, organic solvents like ethanol and acetone have a stimulatory effect on the lipase activity. But, metal ions such as Mg2+, Mn2+, Co2+, Zn2+, Hg2+ and Fe2+ or EDTA, p-chloromercuric benzoate each at 1mM concentration have a profound inhibitory effect on its catalytic activity. These properties together with its stability in organic solvents could form a basis for further characterization of the enzyme as a potential candidate for exploitation at industrial level.
AB - The production of Rhizomucor miehei lipase by solid state fermentation by growing the fungi for 5 days at room temperature on wheat bran medium with a moisture content of 0.75 mL/g is described. The purification of this extracellular enzyme to apparent homogeneity employing a two-step purification sequence using ion-exchange and Octyl-Sepharose affinity chromatography with 135-fold purity and 12% recovery is reported. The purified extracellular enzyme displays maximum activity at pH 7.0 and 65°C. The characteristic feature of this enzyme is its thermostability, retaining 90% of its activity upon exposure to 65°C for 30 min. Further, organic solvents like ethanol and acetone have a stimulatory effect on the lipase activity. But, metal ions such as Mg2+, Mn2+, Co2+, Zn2+, Hg2+ and Fe2+ or EDTA, p-chloromercuric benzoate each at 1mM concentration have a profound inhibitory effect on its catalytic activity. These properties together with its stability in organic solvents could form a basis for further characterization of the enzyme as a potential candidate for exploitation at industrial level.
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M3 - Article
AN - SCOPUS:2242463968
SN - 0022-4456
VL - 57
SP - 607
EP - 610
JO - Journal of Scientific and Industrial Research
JF - Journal of Scientific and Industrial Research
IS - 10-11
ER -