TY - JOUR
T1 - Purification and identification of novel ACE inhibitory and ACE2 upregulating peptides from spent hen muscle proteins
AU - Fan, Hongbing
AU - Wu, Jianping
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2021/5/30
Y1 - 2021/5/30
N2 - The study explored the use of spent hen, a major egg industry byproduct, as the starting material for preparing antihypertensive peptides. While previous studies were focused mainly on ACE inhibitory (ACEi) peptides, this work also studied peptides with ACE2 upregulating (ACE2u) activity, an emerging target for treating hypertension. Spent hen muscle protein hydrolysate prepared by thermoase (SPH-T) exhibited both ACEi and ACE2u activities. After ultrafiltration and chromatographic fractionation, five potent ACEi peptides, VRP, LKY, VRY, KYKA, and LKYKA, with IC50 values of 0.034–5.77 μg/mL, respectively, and four ACE2u peptides, VKW, VHPKESF, VVHPKESF and VAQWRTKYETDAIQRTEELEEAKKK, which increased ACE2 expression by 0.52–0.84 folds, respectively, were identified; VKW also showed ACEi activity. All peptides, except for VRP, are susceptible to degradation during the simulated gastrointestinal digestion. Our study supports the potential use of spent hens as antihypertensive functional food ingredients and nutraceuticals.
AB - The study explored the use of spent hen, a major egg industry byproduct, as the starting material for preparing antihypertensive peptides. While previous studies were focused mainly on ACE inhibitory (ACEi) peptides, this work also studied peptides with ACE2 upregulating (ACE2u) activity, an emerging target for treating hypertension. Spent hen muscle protein hydrolysate prepared by thermoase (SPH-T) exhibited both ACEi and ACE2u activities. After ultrafiltration and chromatographic fractionation, five potent ACEi peptides, VRP, LKY, VRY, KYKA, and LKYKA, with IC50 values of 0.034–5.77 μg/mL, respectively, and four ACE2u peptides, VKW, VHPKESF, VVHPKESF and VAQWRTKYETDAIQRTEELEEAKKK, which increased ACE2 expression by 0.52–0.84 folds, respectively, were identified; VKW also showed ACEi activity. All peptides, except for VRP, are susceptible to degradation during the simulated gastrointestinal digestion. Our study supports the potential use of spent hens as antihypertensive functional food ingredients and nutraceuticals.
KW - ACE
KW - ACE2
KW - Bioactive peptides
KW - Identification
KW - LC-MS/MS
KW - Muscle proteins
KW - Purification
KW - Spent hen
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U2 - 10.1016/j.foodchem.2020.128867
DO - 10.1016/j.foodchem.2020.128867
M3 - Article
C2 - 33352405
AN - SCOPUS:85098451413
SN - 0308-8146
VL - 345
JO - Food Chemistry
JF - Food Chemistry
M1 - 128867
ER -