Purification and identification of novel ACE inhibitory and ACE2 upregulating peptides from spent hen muscle proteins

Hongbing Fan, Jianping Wu

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

The study explored the use of spent hen, a major egg industry byproduct, as the starting material for preparing antihypertensive peptides. While previous studies were focused mainly on ACE inhibitory (ACEi) peptides, this work also studied peptides with ACE2 upregulating (ACE2u) activity, an emerging target for treating hypertension. Spent hen muscle protein hydrolysate prepared by thermoase (SPH-T) exhibited both ACEi and ACE2u activities. After ultrafiltration and chromatographic fractionation, five potent ACEi peptides, VRP, LKY, VRY, KYKA, and LKYKA, with IC50 values of 0.034–5.77 μg/mL, respectively, and four ACE2u peptides, VKW, VHPKESF, VVHPKESF and VAQWRTKYETDAIQRTEELEEAKKK, which increased ACE2 expression by 0.52–0.84 folds, respectively, were identified; VKW also showed ACEi activity. All peptides, except for VRP, are susceptible to degradation during the simulated gastrointestinal digestion. Our study supports the potential use of spent hens as antihypertensive functional food ingredients and nutraceuticals.

Original languageEnglish
Article number128867
JournalFood Chemistry
Volume345
DOIs
StatePublished - May 30 2021

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

  • ACE
  • ACE2
  • Bioactive peptides
  • Identification
  • LC-MS/MS
  • Muscle proteins
  • Purification
  • Spent hen

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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