Abstract
The study explored the use of spent hen, a major egg industry byproduct, as the starting material for preparing antihypertensive peptides. While previous studies were focused mainly on ACE inhibitory (ACEi) peptides, this work also studied peptides with ACE2 upregulating (ACE2u) activity, an emerging target for treating hypertension. Spent hen muscle protein hydrolysate prepared by thermoase (SPH-T) exhibited both ACEi and ACE2u activities. After ultrafiltration and chromatographic fractionation, five potent ACEi peptides, VRP, LKY, VRY, KYKA, and LKYKA, with IC50 values of 0.034–5.77 μg/mL, respectively, and four ACE2u peptides, VKW, VHPKESF, VVHPKESF and VAQWRTKYETDAIQRTEELEEAKKK, which increased ACE2 expression by 0.52–0.84 folds, respectively, were identified; VKW also showed ACEi activity. All peptides, except for VRP, are susceptible to degradation during the simulated gastrointestinal digestion. Our study supports the potential use of spent hens as antihypertensive functional food ingredients and nutraceuticals.
Original language | English |
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Article number | 128867 |
Journal | Food Chemistry |
Volume | 345 |
DOIs | |
State | Published - May 30 2021 |
Bibliographical note
Funding Information:This work was supported by funding from Natural Sciences and Engineering Research Council of Canada, Egg Farmers of Canada and Burnbrae Farms Ltd. H. F. is the recipient of Graduate Student Scholarships from Alberta Innovates Technology Futures and the Killam Trusts.
Funding Information:
This work was supported by funding from Natural Sciences and Engineering Research Council of Canada, Egg Farmers of Canada and Burnbrae Farms Ltd. H. F. is the recipient of Graduate Student Scholarships from Alberta Innovates Technology Futures and the Killam Trusts.
Publisher Copyright:
© 2020 Elsevier Ltd
Keywords
- ACE
- ACE2
- Bioactive peptides
- Identification
- LC-MS/MS
- Muscle proteins
- Purification
- Spent hen
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science