TY - JOUR
T1 - Purification and structure elucidation of the by-product of new regulator of antibiotic production and differentiation of Streptomyces.
AU - Matselyukh, B.
AU - Mohammadipanah, F.
AU - Laatsch, H.
AU - Rohr, J.
AU - Efremenkova, O.
AU - Khilya, V.
PY - 2012
Y1 - 2012
N2 - Streptomyces globisporus 1912, a producer of the antitumor antibiotic landomycin E, forms the new low-molecular signaling molecule N-methylphenylalanyl-dehydrobutyrine diketopiperazine (BDD) and its complex and unstable by-product which restore, like the A-factor in Streptomyces griseus 773, landomycin E and streptomycin biosynthesis, and sporulation of the defective mutants S. globisporus 1912-B2 and S. griseus 1439, respectively. Here, we report the purification and structure elucidation of two compounds with R(f)0.8 by HPLC, LC/MS and 1HMR analysis. These compounds have m/z 338 and 384, accordingly, and each of them is presented by two stereoisomers containing BDD in their structure. A hypothesis explaining the composition and regulatory properties of these unstable compounds is presented.
AB - Streptomyces globisporus 1912, a producer of the antitumor antibiotic landomycin E, forms the new low-molecular signaling molecule N-methylphenylalanyl-dehydrobutyrine diketopiperazine (BDD) and its complex and unstable by-product which restore, like the A-factor in Streptomyces griseus 773, landomycin E and streptomycin biosynthesis, and sporulation of the defective mutants S. globisporus 1912-B2 and S. griseus 1439, respectively. Here, we report the purification and structure elucidation of two compounds with R(f)0.8 by HPLC, LC/MS and 1HMR analysis. These compounds have m/z 338 and 384, accordingly, and each of them is presented by two stereoisomers containing BDD in their structure. A hypothesis explaining the composition and regulatory properties of these unstable compounds is presented.
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M3 - Article
C2 - 23120988
AN - SCOPUS:84871907681
SN - 1028-0987
VL - 74
SP - 66
EP - 73
JO - Mikrobiolohichnyǐ zhurnal (Kiev, Ukraine : 1993)
JF - Mikrobiolohichnyǐ zhurnal (Kiev, Ukraine : 1993)
IS - 5
ER -