Purification and structure elucidation of the by-product of new regulator of antibiotic production and differentiation of Streptomyces.

B. Matselyukh, F. Mohammadipanah, H. Laatsch, J. Rohr, O. Efremenkova, V. Khilya

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Streptomyces globisporus 1912, a producer of the antitumor antibiotic landomycin E, forms the new low-molecular signaling molecule N-methylphenylalanyl-dehydrobutyrine diketopiperazine (BDD) and its complex and unstable by-product which restore, like the A-factor in Streptomyces griseus 773, landomycin E and streptomycin biosynthesis, and sporulation of the defective mutants S. globisporus 1912-B2 and S. griseus 1439, respectively. Here, we report the purification and structure elucidation of two compounds with R(f)0.8 by HPLC, LC/MS and 1HMR analysis. These compounds have m/z 338 and 384, accordingly, and each of them is presented by two stereoisomers containing BDD in their structure. A hypothesis explaining the composition and regulatory properties of these unstable compounds is presented.

Original languageEnglish
Pages (from-to)66-73
Number of pages8
JournalMikrobiolohichnyǐ zhurnal (Kiev, Ukraine : 1993)
Volume74
Issue number5
StatePublished - 2012

ASJC Scopus subject areas

  • General Medicine

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