TY - JOUR
T1 - Quality monitoring of microbial contamination of cryopreserved parathyroid tissue
AU - Stotler, Brie A.
AU - Reich-Slotky, Ronit
AU - Schwartz, Joseph
AU - Inabnet, William B.
AU - Lee, James
AU - Wu, Fann
AU - Della-Latta, Phyllis
AU - Jhang, Jeffrey S.
PY - 2011/5
Y1 - 2011/5
N2 - Cryopreservation of parathyroid tissue (PT) provides patients undergoing parathyroidectomy with an option for delayed autologous heterotopic parathyroid transplantation. A standard protocol for quality monitoring of PT has not been established. This article describes a method for detecting the presence of bacterial contamination in PT tissue intended for autologous transplantation. PT was received in the tissue bank, processed under aseptic conditions, and placed into cryopreservation medium. Sterility testing was performed at 2 time points prior to cryopreservation. From January 2005 to October 2008, 47 PT samples were cryopreserved. The following bacteria were isolated from 11 PT specimens: Staphylococcus epidermidis, Staphylococcus capitis subspecies ureolyticus, Staphylococcus lugdunensis, Bacillus pumilus, and corynebacteria (diphtheroids). 23% of PTs were contaminated at the time of collection, predominantly with indigenous bacteria. Quality monitoring using this protocol is a useful tool to identify tissues contaminated with bacteria.
AB - Cryopreservation of parathyroid tissue (PT) provides patients undergoing parathyroidectomy with an option for delayed autologous heterotopic parathyroid transplantation. A standard protocol for quality monitoring of PT has not been established. This article describes a method for detecting the presence of bacterial contamination in PT tissue intended for autologous transplantation. PT was received in the tissue bank, processed under aseptic conditions, and placed into cryopreservation medium. Sterility testing was performed at 2 time points prior to cryopreservation. From January 2005 to October 2008, 47 PT samples were cryopreserved. The following bacteria were isolated from 11 PT specimens: Staphylococcus epidermidis, Staphylococcus capitis subspecies ureolyticus, Staphylococcus lugdunensis, Bacillus pumilus, and corynebacteria (diphtheroids). 23% of PTs were contaminated at the time of collection, predominantly with indigenous bacteria. Quality monitoring using this protocol is a useful tool to identify tissues contaminated with bacteria.
KW - Autotransplant
KW - Bacteria
KW - Culture
KW - Hypoparathyroidism
KW - Parathyroid
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U2 - 10.1007/s10561-009-9169-5
DO - 10.1007/s10561-009-9169-5
M3 - Article
C2 - 20058088
AN - SCOPUS:79958134205
SN - 1389-9333
VL - 12
SP - 111
EP - 116
JO - Cell and Tissue Banking
JF - Cell and Tissue Banking
IS - 2
ER -