The Borrelia burgdorferi SpoVG protein has previously been found to be a DNA- and RNA-binding protein. To aid in the elucidation of ligand motifs, affinities for numerous RNAs, ssDNAs, and dsDNAs were measured and compared. The loci used in the study were spoVG, glpFKD, erpAB, bb0242, flaB, and ospAB, with particular focus on the untranslated 5′ portion of the mRNAs. Performing binding and competition assays yielded that the 5′ end of spoVG mRNA had the highest affinity while the lowest observed affinity was to the 5’ end of flaB mRNA. Mutagenesis studies of spoVG RNA and ssDNA sequences suggested that the formation of SpoVG-nucleic acid complexes are not entirely dependent on either sequence or structure. Additionally, exchanging uracil for thymine in ssDNAs did not affect protein-nucleic acid complex formation.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Apr 30 2023|
Bibliographical noteFunding Information:
These studies were funded by US NIH grant R01 AI144126 . We thank Tatiana Castro-Padovani and Jessamyn Moore for assistance and helpful comments on this manuscript.
© 2023 The Authors
- Borrelia burgdorferi
- DNA binding protein
- Electrophoretic mobility shift assay (EMSA)
- RNA binding protein
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology