Quantitative analysis of ethyltin compounds in mammalian tissue using hplc/faas

David K. Orren, William M. Brasweil, Paul Mushak

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Measurement of total tin and ethyln tin forms in mammalian tissue is described, using ion-exchange high performance liquid chromatography (HPLC) in tandem with flameless atomic absorption spectrometry (FAAS) for tin-specific detection. All tin forms in whole blood and tissue homogenates were liberated from biological (In vivo) binding by treatment with 6M hydrochloric acid for a period of 4 hr. Ethyln tin species, as the chlorides, were partitioned Into chloroform:ethyl acetate (1:1) and analyzed via HPLC using a strong cation exchange column, with fraction collection by a programmable collector and fraction tin measurement by FAAS. Triethyt- and diethyltin were separated and quantitated using 0.167M ammonium citrate In 70:30 methanokwater, while monoethyltin required the use of 0.50M citrate salt In 70:30 water:methanol as mobile phase to effect elution. The difference between total and speciated tin content provides an estimate of remaining tin species, including the Inorganic element.

Original languageEnglish
Pages (from-to)93-97
Number of pages5
JournalJournal of Analytical Toxicology
Volume10
Issue number3
DOIs
StatePublished - May 1986

Bibliographical note

Funding Information:
This study was supported by the National Institutes of Health, Grant No. ES-01104.

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

Fingerprint

Dive into the research topics of 'Quantitative analysis of ethyltin compounds in mammalian tissue using hplc/faas'. Together they form a unique fingerprint.

Cite this