Abstract
Measurement of total tin and ethyln tin forms in mammalian tissue is described, using ion-exchange high performance liquid chromatography (HPLC) in tandem with flameless atomic absorption spectrometry (FAAS) for tin-specific detection. All tin forms in whole blood and tissue homogenates were liberated from biological (In vivo) binding by treatment with 6M hydrochloric acid for a period of 4 hr. Ethyln tin species, as the chlorides, were partitioned Into chloroform:ethyl acetate (1:1) and analyzed via HPLC using a strong cation exchange column, with fraction collection by a programmable collector and fraction tin measurement by FAAS. Triethyt- and diethyltin were separated and quantitated using 0.167M ammonium citrate In 70:30 methanokwater, while monoethyltin required the use of 0.50M citrate salt In 70:30 water:methanol as mobile phase to effect elution. The difference between total and speciated tin content provides an estimate of remaining tin species, including the Inorganic element.
| Original language | English |
|---|---|
| Pages (from-to) | 93-97 |
| Number of pages | 5 |
| Journal | Journal of Analytical Toxicology |
| Volume | 10 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 1986 |
Bibliographical note
Funding Information:This study was supported by the National Institutes of Health, Grant No. ES-01104.
ASJC Scopus subject areas
- Analytical Chemistry
- Environmental Chemistry
- Toxicology
- Health, Toxicology and Mutagenesis
- Chemical Health and Safety