13 Scopus citations

Abstract

A capillary electrophoresis-laser-induced fluorescence (CE-LIF) method to quantitate reverse transcription-polymerase chain reaction (RT-PCR) products of NAD(P)H:quinone acceptor oxidoreductase (NQO1) derived from whole blood after amplification with a reaction-specific internal standard is reported. The internal standard eliminates variability within the PCR (Hoffman-La Roche, Inc., Nutley, NJ, U.S.A.), while analysis by CE-LIF adds sensitivity and reduces variability associated with isotopic detection. Both the PCR and CE aspects of the assay are precise, with migration time precision of less than 1% and peak area ratio precisions of 9.8-15%. Future applications of this technique may include the analysis of gene therapy, oligonucleotides, and point mutations.

Original languageEnglish
Pages (from-to)287-290
Number of pages4
JournalJournal of capillary electrophoresis
Volume2
Issue number6
StatePublished - 1995

ASJC Scopus subject areas

  • Biochemistry
  • Electrochemistry

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