Abstract
Measurement of total and trimethyl lead in mammalian tissue is described, using ion exchange/high performance liquid chromatography in tandem with flameless atomic absorption spectrometry for lead-specific detection. All lead forms in whole blood and homogenates of soft tissue—brain, kidney, and liver—were liberated from tissue binding by treatment with dilute (3N) HCl for a period of 18 hr. Trimethyl lead was partitioned into chloroform/ethyl acetate after media neutralization to pH of approximately 4 and saturation with sodium chloride. The extract was chromatographically analyzed on a Partisil SCX-10 cation exchange column, using 0.167M ammonium citrate in methanol:water (70:30) as mobile phase. Fractions of eluate were collected using a programmable fraction collector, and the fractions collected from 3.5 to 5.0 min were analyzed by atomic absorption spectrometry. Total lead in tissue was measured by acid wet digestion and flameless atomic absorption spectrometry. The difference in the values from both analyses provided a measure of any trimethyl lead metabolites.
| Original language | English |
|---|---|
| Pages (from-to) | 258-261 |
| Number of pages | 4 |
| Journal | Journal of Analytical Toxicology |
| Volume | 9 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 1985 |
Bibliographical note
Funding Information:"This work was supported by NIH Grant ES-01104 and Training Grant 38616. **Author to whom correspondence should be addressed.
Funding
"This work was supported by NIH Grant ES-01104 and Training Grant 38616. **Author to whom correspondence should be addressed.
| Funders | Funder number |
|---|---|
| National Institutes of Health (NIH) | 38616 |
| National Institute of Environmental Health Sciences (NIEHS) | P01ES001104 |
ASJC Scopus subject areas
- General Medicine