Abstract
Increasing evidence supports a role for altered mitochondrial function in the pathogenesis of neuron degeneration in Alzheimer's disease (AD). Although several studies have examined the effect of amyloid beta peptide (Aβ), on activities of individual proteins in primary neuron cultures, there have been no studies of the effects of Aβ on the mitochondrial proteome. Here, we quantitatively measured changes in mitochondrial proteins of primary rat cortical neuron cultures exposed to 25 μM Aβ25-35 for 16 h using isotope coded affinity tag (ICAT) labeling and 2-dimensional liquid chromatography/tandem mass spectrometry (2D-LC/MS/MS) which allows simultaneous identification and quantification of cysteine-containing proteins. The analysis of enriched mitochondrial fractions identified 10 proteins including sodium/potassium-transporting ATPase, cofilin, dihydropyrimidinase, pyruvate kinase and voltage dependent anion channel 1 that were statistically significantly (P < 0.05) altered in Aβ-treated cultures. Elevations of proteins associated with energy production suggest that cells undergoing Aβ-mediated apoptosis increase synthesis of proteins essential for ATP production and efflux in an attempt to maintain metabolic function.
Original language | English |
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Pages (from-to) | 113-122 |
Number of pages | 10 |
Journal | Neurochemical Research |
Volume | 30 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2005 |
Bibliographical note
Funding Information:This work was supported by National Institutes of Health grants 5P50-AG05144 and 5P01-AG05119 and by a grant from the Abercrombie Foundation. The authors thank Ms. Paula Thomason for editorial assistance.
Keywords
- 2D-LC/MS/MS
- Alzheimer's disease
- Amyloid beta peptide
- ICAT
ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience