Radiation effects on osteoblasts in vitro: A potential role in osteoradionecrosis

T. J. Gal, Teresita Munoz-Antonia, Carlos A. Muro-Cacho, Douglas W. Klotch

Research output: Contribution to journalArticlepeer-review

98 Scopus citations

Abstract

Objective: To evaluate the factors involved in bone remodeling and wound healing that may be altered by radiation therapy. Design: A prospective, controlled study of biochemical activity in vitro. Subjects: MC3T3-E1 mouse osteoblasts. Interventions: Cells were irradiated at 0, 2, 4, or 6 Gy. Specimens were harvested at 1, 7, 14, 28, and 42 days following irradiation for immunohistochemical analysis of transforming growth factor β1 expression and transforming growth factor β1 type I and II receptor expression. Collagen production was measured at 1, 7, 28, 35, and 49 days after irradiation. The effects of dexamethasone on collagen production and cell proliferation were also examined. Results: Irradiated cells demonstrated decreased cell proliferation and a dose-dependent, sustained reduction in collagen production when compared with control cells. An increase in transforming growth factor β1 type I and II receptor expression was noted in irradiated cells when compared with controls. Conclusion: Radiation-induced alterations of factors related to bone remodeling and wound healing have a potential role in the pathogenesis of osteoradionecrosis.

Original languageEnglish
Pages (from-to)1124-1128
Number of pages5
JournalArchives of Otolaryngology—Head and Neck Surgery
Volume126
Issue number9
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

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