Radioimmunoassay of creatine kinase B isoenzyme in human sera: results in patients with acute myocardial infarction

J. T. Willerson, M. J. Stone, R. Ting, A. Mukherjee, C. E. Gomez-Sanchez, P. Lewis, L. B. Hersh

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40 Scopus citations


A radioimmunoassay was developed to measure serum levels of the B isoenzyme of creatine kinase (ATP: creatine N-phosphotransferase, EC (CPK) in order to evaluate the time course and frequency of MB isoenzyme elevation in patients with acute myocardial infarction. The method can identify as little as 0.2 ng of the B portion of the CPK-MB isoenzyme, does not significantly crossreact with CPK-MM isoenzyme, and is not affected by storage of serum at -20°. CPK isoenzyme containing B subunits was detected in 48 out of 51 sera from normal adults; serum levels in these individuals ranged between 1.2 and 12.5 ng/ml [mean±SEM was 2.7±0.30 ng/ml]. The mean serum level of CPK-B isoenzyme in a pool of sera obtained from 100 normal subjects was 2.9±0.35 ng/ml; two patients with rhabdomyolysis that were studied had serum CPK-B isoenzyme levels of 2.5 and 3.5 ng/ml, respectively. In contrast, serum levels of the CPK-B isoenzyme were markedly elevated in sera from 18 patients with acute myocardial infarcts when obtained within 12 hr after hospital admission; the mean ±SEM concentration was 56±7.8 ng/ml. We performed serial determinations on 14 patients with acute myocardial infarctions and demonstrated that maximal serum CPK-B levels occurred within the first 12 hr after admission and were lower thereafter. The serum concentration of B-containing CPK isoenzyme in 19 additional patients admitted with chest pain but without acute myocardial infarction was 3.4±0.50 ng/ml. Thus, radioimmunoassay measurement of CPK-B isoenzyme appears to be a useful and sensitive test for the detection of acute myocardial infarcts in patients.

Original languageEnglish
Pages (from-to)1711-1715
Number of pages5
JournalUnknown Journal
Issue number4
StatePublished - 1977

ASJC Scopus subject areas

  • General


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