RAE1ε Ligand Expressed on Pancreatic Islets Recruits NKG2D Receptor-Expressing Cytotoxic T Cells Independent of T Cell Receptor Recognition

Mary A. Markiewicz; Erica L. Wise; Zachary S. Buchwald; Amelia K. Pinto; Biljana Zafirova; Bojan Polic; Andrey S. Shaw

doi:10.1016/j.immuni.2011.11.014

RAE1ε Ligand Expressed on Pancreatic Islets Recruits NKG2D Receptor-Expressing Cytotoxic T Cells Independent of T Cell Receptor Recognition

Mary A. Markiewicz
, Erica L. Wise
, Zachary S. Buchwald
, Amelia K. Pinto
, Biljana Zafirova
, Bojan Polic
, Andrey S. Shaw

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The mechanisms by which cytotoxic T lymphocytes (CTLs) enter and are retained in nonlymphoid tissue are not well characterized. With a transgenic mouse expressing the NKG2D ligand retinoic acid early transcript 1ε (RAE1ε) in β-islet cells of the pancreas, we found that RAE1 expression was sufficient to induce the recruitment of adoptively transferred CTLs to islets. This was dependent on NKG2D expression by the CTLs and independent of antigen recognition. Surprisingly, the recruitment of CTLs resulted in the subsequent recruitment of a large number of endogenous lymphocytes. Whereas transgenic mice did not develop diabetes, RAE1 expression was sufficient to induce insulitis in older, unmanipulated transgenic mice that was enhanced by viral infection and pancreatic inflammation. These results demonstrate that the expression of an NKG2D ligand in islets is sufficient to recruit CTLs regardless of their antigen specificity and to induce insulitis.

Original language	English
Pages (from-to)	132-141
Number of pages	10
Journal	Immunity
Volume	36
Issue number	1
DOIs	https://doi.org/10.1016/j.immuni.2011.11.014
State	Published - Jan 27 2012

Bibliographical note

Funding Information:
This work was supported by grants from the NIH (Diabetes Research and Training Center P60 DK020579 to M.A.M. and R37 AI057966 to A.S.S.), the HHMI (A.S.S.), and Croatian Ministry of Science, Education and Sports Grant 062-0621261-1271 (B.P.). We thank X. Wang and Y. Huang (Washington University School of Medicine) for their help with performing the BioPlex assays, H. Virgin and D. Kreamalmeyer (Washington University School of Medicine) for mice, and B. Calderon and E. Unanue (Washington University School of Medicine) for helpful advice and discussion.

Funding

This work was supported by grants from the NIH (Diabetes Research and Training Center P60 DK020579 to M.A.M. and R37 AI057966 to A.S.S.), the HHMI (A.S.S.), and Croatian Ministry of Science, Education and Sports Grant 062-0621261-1271 (B.P.). We thank X. Wang and Y. Huang (Washington University School of Medicine) for their help with performing the BioPlex assays, H. Virgin and D. Kreamalmeyer (Washington University School of Medicine) for mice, and B. Calderon and E. Unanue (Washington University School of Medicine) for helpful advice and discussion.

Funders	Funder number
A.S.S.
National Institutes of Health (NIH)
Croatian Ministry of Science, Education and Sports
Diabetes Research and Training Center
B.P. Koirala Institute of Health Sciences
Howard Hughes Medical Institute
National Institute of Diabetes and Digestive and Kidney Diseases	P60DK020579
European Commission	256686
Diabetes Research and Training Center	P60 DK020579, R37 AI057966
Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases	R37AI057966
???publication-publication-funding-organisation-not-added???	062-0621261-1271

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Infectious Diseases

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10.1016/j.immuni.2011.11.014

Cite this

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title = "RAE1ε Ligand Expressed on Pancreatic Islets Recruits NKG2D Receptor-Expressing Cytotoxic T Cells Independent of T Cell Receptor Recognition",

abstract = "The mechanisms by which cytotoxic T lymphocytes (CTLs) enter and are retained in nonlymphoid tissue are not well characterized. With a transgenic mouse expressing the NKG2D ligand retinoic acid early transcript 1ε (RAE1ε) in β-islet cells of the pancreas, we found that RAE1 expression was sufficient to induce the recruitment of adoptively transferred CTLs to islets. This was dependent on NKG2D expression by the CTLs and independent of antigen recognition. Surprisingly, the recruitment of CTLs resulted in the subsequent recruitment of a large number of endogenous lymphocytes. Whereas transgenic mice did not develop diabetes, RAE1 expression was sufficient to induce insulitis in older, unmanipulated transgenic mice that was enhanced by viral infection and pancreatic inflammation. These results demonstrate that the expression of an NKG2D ligand in islets is sufficient to recruit CTLs regardless of their antigen specificity and to induce insulitis.",

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note = "Funding Information: This work was supported by grants from the NIH (Diabetes Research and Training Center P60 DK020579 to M.A.M. and R37 AI057966 to A.S.S.), the HHMI (A.S.S.), and Croatian Ministry of Science, Education and Sports Grant 062-0621261-1271 (B.P.). We thank X. Wang and Y. Huang (Washington University School of Medicine) for their help with performing the BioPlex assays, H. Virgin and D. Kreamalmeyer (Washington University School of Medicine) for mice, and B. Calderon and E. Unanue (Washington University School of Medicine) for helpful advice and discussion. ",

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AU - Shaw, Andrey S.

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RAE1ε Ligand Expressed on Pancreatic Islets Recruits NKG2D Receptor-Expressing Cytotoxic T Cells Independent of T Cell Receptor Recognition

Abstract

Bibliographical note

Funding

UN SDGs

ASJC Scopus subject areas

Access to Document

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