TY - JOUR
T1 - RAKE and LNA-ISH reveal microRNA expression and localization in archival human brain
AU - Nelson, Peter T.
AU - Baldwin, Don A.
AU - Kloosterman, Wigard P.
AU - Kauppinen, Sakari
AU - Plasterk, Ronald H.A.
AU - Mourelatos, Zissimos
PY - 2006/2
Y1 - 2006/2
N2 - microRNAs (miRNAs) are small (∼22 nucleotide) regulatory RNAs which play fundamental roles in many biological processes. Recent studies have shown that the expression of many miRNAs is altered in various human tumors and some miRNAs may function as oncogenes or tumor suppressor genes. However, with the exception of glioblastoma multiforme, the expression of miRNAs in brain tumors is unknown. Furthermore, methods to profile miRNAs from formalin-fixed, paraffin-embedded (FFPE) archival tissues or to study their cellular and subcellular localization in FFPE tissues have been lacking. Here we report the coordinated miRNA expression analysis from the tissue level to the subcellular level, using the RAKE (RNA-primed, array-based, Klenow Enzyme) miRNA microarray platform in conjunction with Locked Nucleic Acid (LNA)-based in situ hybridization (LNA-ISH) on archival FFPE human brains and oligodendroglial tumors. The ability to profile miRNAs from archival tissues at the tissue level, by RAKE microarrays, and at the cellular level by LNA-ISH, will accelerate studies of miRNAs in human diseases. Published by Cold Spring Harbor Laboratory Press.
AB - microRNAs (miRNAs) are small (∼22 nucleotide) regulatory RNAs which play fundamental roles in many biological processes. Recent studies have shown that the expression of many miRNAs is altered in various human tumors and some miRNAs may function as oncogenes or tumor suppressor genes. However, with the exception of glioblastoma multiforme, the expression of miRNAs in brain tumors is unknown. Furthermore, methods to profile miRNAs from formalin-fixed, paraffin-embedded (FFPE) archival tissues or to study their cellular and subcellular localization in FFPE tissues have been lacking. Here we report the coordinated miRNA expression analysis from the tissue level to the subcellular level, using the RAKE (RNA-primed, array-based, Klenow Enzyme) miRNA microarray platform in conjunction with Locked Nucleic Acid (LNA)-based in situ hybridization (LNA-ISH) on archival FFPE human brains and oligodendroglial tumors. The ability to profile miRNAs from archival tissues at the tissue level, by RAKE microarrays, and at the cellular level by LNA-ISH, will accelerate studies of miRNAs in human diseases. Published by Cold Spring Harbor Laboratory Press.
KW - Brain tumors
KW - In situ hybridization
KW - Locked nucleic acid
KW - MicroRNA
KW - RAKE
KW - miRNA
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UR - http://www.scopus.com/inward/citedby.url?scp=31044455031&partnerID=8YFLogxK
U2 - 10.1261/rna.2258506
DO - 10.1261/rna.2258506
M3 - Article
C2 - 16373485
AN - SCOPUS:31044455031
SN - 1355-8382
VL - 12
SP - 187
EP - 191
JO - RNA
JF - RNA
IS - 2
ER -
