Range-wide population genomics of the Mexican fruit fly: Toward development of pathway analysis tools

Julian R. Dupuis, Raul Ruiz-Arce, Norman B. Barr, Donald B. Thomas, Scott M. Geib

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Recurrently invading pests provide unique challenges for pest management, but also present opportunities to utilize genomics to understand invasion dynamics and inform regulatory management through pathway analysis. In the southern United States, the Mexican fruit fly Anastrepha ludens is such a pest, and its incursions into Texas and California represent major threats to the agricultural systems of those regions. We developed a draft genome assembly for A. ludens, conducted range-wide population genomics using restriction site-associated DNA sequencing, and then developed and demonstrated a panel of highly differentiated diagnostic SNPs for source determination of intercepted flies in this system. Using 2,081 genomewide SNPs, we identified four populations across the range of A. ludens, corresponding to western Mexico, eastern Mexico/Texas, Guatemala/Belize/Honduras, and Costa Rica/Panama, with some intergradation present between clusters, particularly in Central America. From this population genomics framework, we developed a diagnostic panel of 28 highly differentiated SNPs that were able to recreate the genomewide population structure in this species. We demonstrated this panel on a set of test specimens, including specimens intercepted as part of regular trapping surveillance in Texas and California, and we were able to predict populations of origin for these specimens. This methodology presents a highly applied use of genomic techniques and can be implemented in any group of recurrently invading pests.

Original languageEnglish
Pages (from-to)1641-1660
Number of pages20
JournalEvolutionary Applications
Volume12
Issue number8
DOIs
StatePublished - 2019

Bibliographical note

Publisher Copyright:
© 2019 The Authors. Evolutionary Applications published by John Wiley & Sons Ltd

Keywords

  • Anastrepha
  • Fluidigm
  • Tephritidae
  • agricultural pest
  • ddRAD
  • pathway analysis
  • source determination

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • General Agricultural and Biological Sciences

Fingerprint

Dive into the research topics of 'Range-wide population genomics of the Mexican fruit fly: Toward development of pathway analysis tools'. Together they form a unique fingerprint.

Cite this