Rapid and direct action of lipopolysaccharide (Lps) on skeletal muscle of larval drosophila

Rachel Potter, Alexis Meade, Samuel Potter, Robin L. Cooper

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The endotoxin lipopolysaccharide (LPS) from Gram-negative bacteria exerts a direct and rapid effect on tissues. While most attention is given to the downstream actions of the immune system in response to LPS, this study focuses on the direct actions of LPS on skeletal muscle in Drosophila melanogaster. It was noted in earlier studies that the membrane potential rapidly hyperpolarizes in a dose-dependent manner with exposure to LPS from Pseudomonas aeruginosa and Serratia marcescens. The response is transitory while exposed to LPS, and the effect does not appear to be due to calcium-activated potassium channels, activated nitric oxide synthase (NOS), or the opening of Cl channels. The purpose of this study was to further investigate the mechanism of the hyperpolarization of the larval Drosophila muscle due to exposure of LPS using several different experimental paradigms. It appears this response is unlikely related to activation of the Na-K pump or Ca2+ influx. The unknown activation of a K+ efflux could be responsible. This will be an important factor to consider in treatments of bacterial septicemia and cellular energy demands.

Original languageEnglish
Article number1235
Issue number12
StatePublished - Dec 2021

Bibliographical note

Funding Information:
was provided by Chellgren Endowed Funding to R.L.C. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.


  • Endotoxin
  • Insect
  • Na-K pump
  • Ouabain
  • Potassium channel

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)
  • Immunology and Microbiology (all)
  • Agricultural and Biological Sciences (all)


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