Rapid assessment of salivary MMP-8 and periodontal disease using lateral flow immunoassay

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57 Scopus citations


Objective: The objective of this study was to determine the efficacy of a novel point-of-care immunoflow device (POCID) for detecting matrix metalloproteinase (MMP)-8 concentrations in oral fluids in comparison with a gold standard laboratory-based immunoassay. Methods: Oral rinse fluid and whole expectorated saliva samples were collected from 41 participants clinically classified as periodontally healthy or diseased. Samples were analyzed for MMP-8 by Luminex immunoassay and POCID. Photographed POCID results were assessed by optical scan and visually by two examiners. Data were analyzed by Pearson's correlation and receiver-operating characteristics. Results: MMP-8 was readily detected by the POCID, and concentrations correlated well with Luminex for both saliva and rinse fluids (r = 0.57–0.93). Thresholds that distinguished periodontitis from health were delineated from both the optical scans and visual reads of the POCID (sensitivity: 0.7–0.9, specificity: 0.5–0.7; P < 0.05). Conclusions: Performance of this POCID for detecting MMP-8 in oral rinse fluid or saliva was excellent. These findings help demonstrate the utility of salivary biomarkers for distinguishing periodontal disease from health using a rapid point-of-care approach.

Original languageEnglish
Pages (from-to)681-687
Number of pages7
JournalOral Diseases
Issue number7
StatePublished - Oct 1 2016

Bibliographical note

Publisher Copyright:
© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd


  • MMP-8
  • biomarker
  • periodontitis
  • point-of-care
  • saliva

ASJC Scopus subject areas

  • Otorhinolaryngology
  • General Dentistry


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