Rapid estimation of potato tuber total protein content with coomassie brilliant blue G-250

J. C. Snyder; S. L. Desborough

doi:10.1007/BF00264747

Rapid estimation of potato tuber total protein content with coomassie brilliant blue G-250

J. C. Snyder, S. L. Desborough

Horticulture

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

A new method for measuring protein with Coo-massie Brilliant Blue G-250 has been adapted for use as a screening method in a potato tuber protein improvement breeding program. The method is simple, fast and inexpensive, and has successfully estimated the total protein content of a broad range of tuber genotypes having dissimilar amino acid profiles and tuber maturities. Correlation between the Coomassie method and a modified micro-Kjeldahl method, the standard method used in the potato breeding program, was 0.93. Free amino acids and other compounds which interfere with other methods for measuring protein do not interfere with the Coomassie Brilliant Blue procedure.

Original language	English
Pages (from-to)	135-139
Number of pages	5
Journal	Theoretical And Applied Genetics
Volume	52
Issue number	3
DOIs	https://doi.org/10.1007/BF00264747
State	Published - May 1978

Keywords

Protein Breeding
Protein Dye-Binding
Protein Extraction
Protein Selection Method
Solanum tuberosum L.

ASJC Scopus subject areas

Biotechnology
Agronomy and Crop Science
Genetics

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10.1007/BF00264747

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title = "Rapid estimation of potato tuber total protein content with coomassie brilliant blue G-250",

abstract = "A new method for measuring protein with Coo-massie Brilliant Blue G-250 has been adapted for use as a screening method in a potato tuber protein improvement breeding program. The method is simple, fast and inexpensive, and has successfully estimated the total protein content of a broad range of tuber genotypes having dissimilar amino acid profiles and tuber maturities. Correlation between the Coomassie method and a modified micro-Kjeldahl method, the standard method used in the potato breeding program, was 0.93. Free amino acids and other compounds which interfere with other methods for measuring protein do not interfere with the Coomassie Brilliant Blue procedure.",

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N2 - A new method for measuring protein with Coo-massie Brilliant Blue G-250 has been adapted for use as a screening method in a potato tuber protein improvement breeding program. The method is simple, fast and inexpensive, and has successfully estimated the total protein content of a broad range of tuber genotypes having dissimilar amino acid profiles and tuber maturities. Correlation between the Coomassie method and a modified micro-Kjeldahl method, the standard method used in the potato breeding program, was 0.93. Free amino acids and other compounds which interfere with other methods for measuring protein do not interfere with the Coomassie Brilliant Blue procedure.

AB - A new method for measuring protein with Coo-massie Brilliant Blue G-250 has been adapted for use as a screening method in a potato tuber protein improvement breeding program. The method is simple, fast and inexpensive, and has successfully estimated the total protein content of a broad range of tuber genotypes having dissimilar amino acid profiles and tuber maturities. Correlation between the Coomassie method and a modified micro-Kjeldahl method, the standard method used in the potato breeding program, was 0.93. Free amino acids and other compounds which interfere with other methods for measuring protein do not interfere with the Coomassie Brilliant Blue procedure.

KW - Protein Breeding

KW - Protein Dye-Binding

KW - Protein Extraction

KW - Protein Selection Method

KW - Solanum tuberosum L.

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Rapid estimation of potato tuber total protein content with coomassie brilliant blue G-250

Abstract

Keywords

ASJC Scopus subject areas

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