Rapid estimation of potato tuber total protein content with coomassie brilliant blue G-250

J. C. Snyder, S. L. Desborough

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

A new method for measuring protein with Coo-massie Brilliant Blue G-250 has been adapted for use as a screening method in a potato tuber protein improvement breeding program. The method is simple, fast and inexpensive, and has successfully estimated the total protein content of a broad range of tuber genotypes having dissimilar amino acid profiles and tuber maturities. Correlation between the Coomassie method and a modified micro-Kjeldahl method, the standard method used in the potato breeding program, was 0.93. Free amino acids and other compounds which interfere with other methods for measuring protein do not interfere with the Coomassie Brilliant Blue procedure.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalTheoretical And Applied Genetics
Volume52
Issue number3
DOIs
StatePublished - May 1978

Keywords

  • Protein Breeding
  • Protein Dye-Binding
  • Protein Extraction
  • Protein Selection Method
  • Solanum tuberosum L.

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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