Rapid fabrication of precise high-throughput filters from membrane protein nanosheets

Yu Ming Tu, Woochul Song, Tingwei Ren, Yue xiao Shen, Ratul Chowdhury, Prasangi Rajapaksha, Tyler E. Culp, Laxmicharan Samineni, Chao Lang, Alina Thokkadam, Drew Carson, Yuxuan Dai, Arwa Mukthar, Miaoci Zhang, Andrey Parshin, Janna N. Sloand, Scott H. Medina, Mariusz Grzelakowski, Dibakar Bhattacharya, William A. PhillipEnrique D. Gomez, Robert J. Hickey, Yinai Wei, Manish Kumar

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


Biological membranes are ideal for separations as they provide high permeability while maintaining high solute selectivity due to the presence of specialized membrane protein (MP) channels. However, successful integration of MPs into manufactured membranes has remained a significant challenge. Here, we demonstrate a two-hour organic solvent method to develop 2D crystals and nanosheets of highly packed pore-forming MPs in block copolymers (BCPs). We then integrate these hybrid materials into scalable MP-BCP biomimetic membranes. These MP-BCP nanosheet membranes maintain the molecular selectivity of the three types of β-barrel MP channels used, with pore sizes of 0.8 nm, 1.3 nm, and 1.5 nm. These biomimetic membranes demonstrate water permeability that is 20–1,000 times greater than that of commercial membranes and 1.5–45 times greater than that of the latest research membranes with comparable molecular exclusion ratings. This approach could provide high performance alternatives in the challenging sub-nanometre to few-nanometre size range.

Original languageEnglish
Pages (from-to)347-354
Number of pages8
JournalNature Materials
Issue number3
StatePublished - Mar 1 2020

Bibliographical note

Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature Limited.

ASJC Scopus subject areas

  • General Chemistry
  • General Materials Science
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering


Dive into the research topics of 'Rapid fabrication of precise high-throughput filters from membrane protein nanosheets'. Together they form a unique fingerprint.

Cite this