Rapid in vitro multiplication of non-runnering fragaria vesca genotypes from seedling shoot axillary bud explants

Babul C. Sarker, Douglas D. Archbold, Robert L. Geneve, Sharon T. Kester

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Fragaria vesca L. has become a model species for genomic studies relevant to important crop plant species in the Rosaceae family, but generating large numbers of plants from non-runner-producing genotypes is slow. To develop a protocol for the rapid generation of plants, leaf explants were compared to single axillary bud shoot explants, both from in vitro-grown Fragaria vesca seedlings, as sources of shoots for new plant production in response to benzyladenine (BA) or thidiazuron (TDZ) combined with indolebutyric acid (IBA) on Murashige and Skoog’s Basal Salt (MS) medium. BA at 2.0 and 4.0 mg L−1 and TDZ at 1.5 mg L−1 promoted the greatest number of shoots produced per shoot explant. There were no IBA effects or IBA interactions with BA or TDZ. Significant interactions between BA and IBA, but not TDZ and IBA, occurred in leaf explant callus formation and % explants with callus at 6 and 9 weeks of culture and on shoots per leaf explant at 9 weeks. TDZ treatments produced uniformly high levels of callus but low numbers of shoots. The treatment generating the most shoot production was BA at 4.0 mg L−1 plus IBA at 0.50 mg L−1 . After 9 weeks of culture, leaf explants of the non-runner-producing genotype Baron Solemacher had generated 4.6 shoots per explant with the best treatment, while axillary bud explants had generated 30.8 shoots with the best treatment. Thus, in vitro culture of shoot axillary bud explants can generate high numbers of clonal shoots from a single seedling plant in vitro.

Original languageEnglish
Article number51
Pages (from-to)1-8
Number of pages8
JournalHorticulturae
Volume6
Issue number3
DOIs
StatePublished - Sep 2020

Bibliographical note

Funding Information:
Acknowledgments: This is publication No. 17-11-064 of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. Babul C. Sarker would like to express his gratitude to the Fulbright authority (CIES/IIE) for providing a Fellowship to carry out research under the Fulbright Scholars Program at the Department of Horticulture, University of Kentucky, Lexington, Kentucky, USA. Technical as well as other support and cooperation by numerous faculty and staff of the Department of Horticulture, University of Kentucky, Lexington, Kentucky, USA are gratefully acknowledged. Thanks to the Bangladesh Agricultural Research Institute, Gazipur, Bangladesh, for approving leave for the period of study and research. This project was supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch project number KY011042, and the Fulbright Scholars Program.

Funding Information:
Fulbright authority (CIES/IIE) provided the fellowship under Fulbright Visiting Scholar Program to perform the research. Acknowledgments: This is publication No. 17-11-064 of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. Babul C. Sarker would like to express his gratitude to the Fulbright authority (CIES/IIE) for providing a Fellowship to carry out research under the Fulbright Scholars Program at the Department of Horticulture, University of Kentucky, Lexington, Kentucky, USA. Technical as well as other support and cooperation by numerous faculty and staff of the Department of Horticulture, University of Kentucky, Lexington, Kentucky, USA are gratefully acknowledged. Thanks to the Bangladesh Agricultural Research Institute, Gazipur, Bangladesh, for approving leave for the period of study and research. This project was supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch project number KY011042, and the Fulbright Scholars Program.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Alpine strawberry
  • BA
  • IBA
  • In vitro multiplication
  • Non-runnering
  • Shoot explant
  • TDZ

ASJC Scopus subject areas

  • Plant Science
  • Horticulture

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