Rapid isolation and identification of bacteriophage T4-Encoded modifications of Escherichia coli RNA polymerase: A generic method to study Bacteriophage/Host interactions

F. Westblade Lars; Leonid Minakhin; Konstantin Kuznedelov; Alan J. Tackett; Emmanuel J. Chang; Rachel A. Mooney; Irina Vvedenskaya; Qing Jun Wang; David Fenyö; Michael P. Rout; Robert Landick; Brian T. Chait; Konstantin Severinov; Seth A. Darst

doi:10.1021/pr070451j

Rapid isolation and identification of bacteriophage T4-Encoded modifications of Escherichia coli RNA polymerase: A generic method to study Bacteriophage/Host interactions

F. Westblade Lars, Leonid Minakhin, Konstantin Kuznedelov, Alan J. Tackett, Emmanuel J. Chang, Rachel A. Mooney, Irina Vvedenskaya, Qing Jun Wang, David Fenyö, Michael P. Rout, Robert Landick, Brian T. Chait, Konstantin Severinov, Seth A. Darst

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriaphage-dependent alterations of the host synthesis machinery.

Original language	English
Pages (from-to)	1244-1250
Number of pages	7
Journal	Journal of Proteome Research
Volume	7
Issue number	3
DOIs	https://doi.org/10.1021/pr070451j
State	Published - Mar 2008

Keywords

Affinityisolation
Bacteriophage
Bacteriophage infection
Coli
Escherichia
MALDI MS
RNA polymerase

ASJC Scopus subject areas

General Chemistry
Biochemistry

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10.1021/pr070451j

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Lars, F. W., Minakhin, L., Kuznedelov, K., Tackett, A. J., Chang, E. J., Mooney, R. A., Vvedenskaya, I., Wang, Q. J., Fenyö, D., Rout, M. P., Landick, R., Chait, B. T., Severinov, K., & Darst, S. A. (2008). Rapid isolation and identification of bacteriophage T4-Encoded modifications of Escherichia coli RNA polymerase: A generic method to study Bacteriophage/Host interactions. Journal of Proteome Research, 7(3), 1244-1250. https://doi.org/10.1021/pr070451j

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title = "Rapid isolation and identification of bacteriophage T4-Encoded modifications of Escherichia coli RNA polymerase: A generic method to study Bacteriophage/Host interactions",

abstract = "Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriaphage-dependent alterations of the host synthesis machinery.",

keywords = "Affinityisolation, Bacteriophage, Bacteriophage infection, Coli, Escherichia, MALDI MS, RNA polymerase",

author = "Lars, {F. Westblade} and Leonid Minakhin and Konstantin Kuznedelov and Tackett, {Alan J.} and Chang, {Emmanuel J.} and Mooney, {Rachel A.} and Irina Vvedenskaya and Wang, {Qing Jun} and David Feny{\"o} and Rout, {Michael P.} and Robert Landick and Chait, {Brian T.} and Konstantin Severinov and Darst, {Seth A.}",

year = "2008",

month = mar,

doi = "10.1021/pr070451j",

language = "English",

volume = "7",

pages = "1244--1250",

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Lars, FW, Minakhin, L, Kuznedelov, K, Tackett, AJ, Chang, EJ, Mooney, RA, Vvedenskaya, I, Wang, QJ, Fenyö, D, Rout, MP, Landick, R, Chait, BT, Severinov, K & Darst, SA 2008, 'Rapid isolation and identification of bacteriophage T4-Encoded modifications of Escherichia coli RNA polymerase: A generic method to study Bacteriophage/Host interactions', Journal of Proteome Research, vol. 7, no. 3, pp. 1244-1250. https://doi.org/10.1021/pr070451j

Rapid isolation and identification of bacteriophage T4-Encoded modifications of Escherichia coli RNA polymerase: A generic method to study Bacteriophage/Host interactions. / Lars, F. Westblade; Minakhin, Leonid; Kuznedelov, Konstantin et al.
In: Journal of Proteome Research, Vol. 7, No. 3, 03.2008, p. 1244-1250.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Rapid isolation and identification of bacteriophage T4-Encoded modifications of Escherichia coli RNA polymerase

T2 - A generic method to study Bacteriophage/Host interactions

AU - Lars, F. Westblade

AU - Minakhin, Leonid

AU - Kuznedelov, Konstantin

AU - Tackett, Alan J.

AU - Chang, Emmanuel J.

AU - Mooney, Rachel A.

AU - Vvedenskaya, Irina

AU - Wang, Qing Jun

AU - Fenyö, David

AU - Rout, Michael P.

AU - Landick, Robert

AU - Chait, Brian T.

AU - Severinov, Konstantin

AU - Darst, Seth A.

PY - 2008/3

Y1 - 2008/3

N2 - Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriaphage-dependent alterations of the host synthesis machinery.

AB - Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriaphage-dependent alterations of the host synthesis machinery.

KW - Affinityisolation

KW - Bacteriophage

KW - Bacteriophage infection

KW - Coli

KW - Escherichia

KW - MALDI MS

KW - RNA polymerase

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U2 - 10.1021/pr070451j

DO - 10.1021/pr070451j

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AN - SCOPUS:44449102824

SN - 1535-3893

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SP - 1244

EP - 1250

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 3

ER -

Rapid isolation and identification of bacteriophage T4-Encoded modifications of Escherichia coli RNA polymerase: A generic method to study Bacteriophage/Host interactions

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