TY - GEN
T1 - Rapid nucleic acid purification via microchannel immiscible phase filtration
AU - Berry, Scott M.
AU - Beebe, David J.
PY - 2010
Y1 - 2010
N2 - Quality sample preparation is an important prerequisite for downstream analysis of nucleic acids (NAs). Currently, this ubiquitous process requires a significant fraction of the available workload within many life science laboratories. In this manuscript, we expedite NA sample purification by utilizing an immiscible phase to filter contaminants (e.g. proteins, cell debris, lysis buffer) in a single step. In this process, paramagnetic particles (PMPs) which selectively bind NAs are magnetically driven across an immiscible barrier, termed the Phase-Gate. Aided by the surface tension-dominant physics associated with the microscale, this technique reduces purification time by ∼67% without sacrificing other aspects of performance.
AB - Quality sample preparation is an important prerequisite for downstream analysis of nucleic acids (NAs). Currently, this ubiquitous process requires a significant fraction of the available workload within many life science laboratories. In this manuscript, we expedite NA sample purification by utilizing an immiscible phase to filter contaminants (e.g. proteins, cell debris, lysis buffer) in a single step. In this process, paramagnetic particles (PMPs) which selectively bind NAs are magnetically driven across an immiscible barrier, termed the Phase-Gate. Aided by the surface tension-dominant physics associated with the microscale, this technique reduces purification time by ∼67% without sacrificing other aspects of performance.
KW - Microfluidics
KW - Nucleic acids
KW - PCR
KW - Sample preparation
UR - http://www.scopus.com/inward/record.url?scp=84884315243&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884315243&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884315243
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 665
EP - 667
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -