Reaction of vanadium(V) with thiols generates vanadium(IV) and thiyl radicals

Xianglin Shi; Xiaoya Sun; N. S. Dalal

doi:10.1016/0014-5793(90)80402-5

Reaction of vanadium(V) with thiols generates vanadium(IV) and thiyl radicals

Xianglin Shi, Xiaoya Sun, N. S. Dalal

Toxicology and Cancer Biology

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

The in vivo toxicity of vanadium(V) has been found to correlate with the depletion of cellular glutathione and related non-protein thiols. With a view to understanding the mechanism for this observation, we have investigated the oxidation of glutathione, cysteine N-acetylcysteine and penicillamine by vanadium(V), using electron spin resonance (ESR) and ESR spin trapping methodology. The spin trap used was 5,5-dimethyl-1-pyrroline 1-oxide (DMPO). It is found that the oxidation of these thiols by vanadium(V) generates the corresponding thiyl radicals and vanadium- (IV) complexes. The results suggest that free radical reactions play a significant role in the depletion of cellular thiols by vanadium(V) and hence in vanadium(V) toxicity.

Original language	English
Pages (from-to)	185-188
Number of pages	4
Journal	FEBS Letters
Volume	271
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(90)80402-5
State	Published - Oct 1 1990

Bibliographical note

Funding Information:
~ck~o~/e~ge~e~ts: Part of this research has been supported by the Department of the Interior’s Mineral Institute program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under Grant Gi 135142.

Keywords

ESR
Non-protein thiol
Spin trapping
Thiyl radical
Vanadium toxicity
Vanadium(V)

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(90)80402-5

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title = "Reaction of vanadium(V) with thiols generates vanadium(IV) and thiyl radicals",

abstract = "The in vivo toxicity of vanadium(V) has been found to correlate with the depletion of cellular glutathione and related non-protein thiols. With a view to understanding the mechanism for this observation, we have investigated the oxidation of glutathione, cysteine N-acetylcysteine and penicillamine by vanadium(V), using electron spin resonance (ESR) and ESR spin trapping methodology. The spin trap used was 5,5-dimethyl-1-pyrroline 1-oxide (DMPO). It is found that the oxidation of these thiols by vanadium(V) generates the corresponding thiyl radicals and vanadium- (IV) complexes. The results suggest that free radical reactions play a significant role in the depletion of cellular thiols by vanadium(V) and hence in vanadium(V) toxicity.",

keywords = "ESR, Non-protein thiol, Spin trapping, Thiyl radical, Vanadium toxicity, Vanadium(V)",

author = "Xianglin Shi and Xiaoya Sun and Dalal, {N. S.}",

note = "Funding Information: ~ck~o~/e~ge~e~ts: Part of this research has been supported by the Department of the Interior{\textquoteright}s Mineral Institute program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under Grant Gi 135142.",

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doi = "10.1016/0014-5793(90)80402-5",

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AU - Shi, Xianglin

AU - Sun, Xiaoya

AU - Dalal, N. S.

N1 - Funding Information: ~ck~o~/e~ge~e~ts: Part of this research has been supported by the Department of the Interior’s Mineral Institute program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under Grant Gi 135142.

PY - 1990/10/1

Y1 - 1990/10/1

N2 - The in vivo toxicity of vanadium(V) has been found to correlate with the depletion of cellular glutathione and related non-protein thiols. With a view to understanding the mechanism for this observation, we have investigated the oxidation of glutathione, cysteine N-acetylcysteine and penicillamine by vanadium(V), using electron spin resonance (ESR) and ESR spin trapping methodology. The spin trap used was 5,5-dimethyl-1-pyrroline 1-oxide (DMPO). It is found that the oxidation of these thiols by vanadium(V) generates the corresponding thiyl radicals and vanadium- (IV) complexes. The results suggest that free radical reactions play a significant role in the depletion of cellular thiols by vanadium(V) and hence in vanadium(V) toxicity.

AB - The in vivo toxicity of vanadium(V) has been found to correlate with the depletion of cellular glutathione and related non-protein thiols. With a view to understanding the mechanism for this observation, we have investigated the oxidation of glutathione, cysteine N-acetylcysteine and penicillamine by vanadium(V), using electron spin resonance (ESR) and ESR spin trapping methodology. The spin trap used was 5,5-dimethyl-1-pyrroline 1-oxide (DMPO). It is found that the oxidation of these thiols by vanadium(V) generates the corresponding thiyl radicals and vanadium- (IV) complexes. The results suggest that free radical reactions play a significant role in the depletion of cellular thiols by vanadium(V) and hence in vanadium(V) toxicity.

KW - ESR

KW - Non-protein thiol

KW - Spin trapping

KW - Thiyl radical

KW - Vanadium toxicity

KW - Vanadium(V)

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JO - FEBS Letters

JF - FEBS Letters

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Reaction of vanadium(V) with thiols generates vanadium(IV) and thiyl radicals

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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