Reactor performance and microbial community dynamics during solid-state anaerobic digestion of corn stover at mesophilic and thermophilic conditions

Jian Shi, Zhongjiang Wang, Jill A. Stiverson, Zhongtang Yu, Yebo Li

Research output: Contribution to journalArticlepeer-review

122 Scopus citations

Abstract

Reactor performance and microbial community dynamics were investigated during solid state anaerobic digestion (SS-AD) of corn stover at mesophilic and thermophilic conditions. Thermophilic SS-AD led to faster and greater reductions of cellulose and hemicelluloses during the first 12. days compared to mesophilic SS-AD. However, accumulation of volatile fatty acids (VFAs) was 5-fold higher at thermophilic than mesophilic temperatures, resulting in a large pH drop during days 6-12 in the thermophilic reactors. Culture-based enumeration revealed 10-50 times greater populations of cellulolytic and xylanolytic microbes during thermophilic SS-AD than mesophilic SS-AD. DGGE analysis of PCR amplified 16S rRNA genes showed dynamic shifts, especially during the thermophilic SS-AD, of bacterial and archaeal communities over the 38. days of SS-AD as a result of acclimation of the initial seed microbial consortia to the lignocellulosic feedstock. The findings of this study can guide future studies to improve efficiency and stability of SS-AD.

Original languageEnglish
Pages (from-to)574-581
Number of pages8
JournalBioresource Technology
Volume136
DOIs
StatePublished - May 2013

Keywords

  • 16S rRNA
  • Anaerobic digestion
  • Biogas
  • Denaturing gradient gel electrophoresis
  • Solid state

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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