Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography

Ahmad A. Salaimeh, Jeffrey J. Campion, Belal Y. Gharaibeh, Martin E. Evans, Kozo Saito

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia coli, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to ∼4 × 10 8 CFU/ml. The EC of S. aureus was ∼2-fold higher than that of E. coli at comparable cell concentrations suggesting that IRT may be used to distinguish genera.

Original languageEnglish
Pages (from-to)170-172
Number of pages3
JournalInfrared Physics and Technology
Issue number1
StatePublished - Jan 2012

Bibliographical note

Funding Information:
We would like to thank Judy Glass for help with the experiments, and Mary Lou Cahal for assistance in preparing the figures. This study was supported in part by a fundamental research grant from the Institute of Research for Technology Development (IR4TD), University of Kentucky and with use of laboratory facilities at the Lexington Veterans Affairs Medical Center, Lexington, Kentucky.


  • Bacterial quantification
  • Energy content
  • Infrared thermography
  • Real-time
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics


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