We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia coli, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to ∼4 × 10 8 CFU/ml. The EC of S. aureus was ∼2-fold higher than that of E. coli at comparable cell concentrations suggesting that IRT may be used to distinguish genera.
|Number of pages||3|
|Journal||Infrared Physics and Technology|
|State||Published - Jan 2012|
Bibliographical noteFunding Information:
We would like to thank Judy Glass for help with the experiments, and Mary Lou Cahal for assistance in preparing the figures. This study was supported in part by a fundamental research grant from the Institute of Research for Technology Development (IR4TD), University of Kentucky and with use of laboratory facilities at the Lexington Veterans Affairs Medical Center, Lexington, Kentucky.
- Bacterial quantification
- Energy content
- Infrared thermography
- Staphylococcus aureus
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Condensed Matter Physics