Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography

Ahmad A. Salaimeh, Jeffrey J. Campion, Belal Y. Gharaibeh, Martin E. Evans, Kozo Saito

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia coli, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to ∼4 × 10 8 CFU/ml. The EC of S. aureus was ∼2-fold higher than that of E. coli at comparable cell concentrations suggesting that IRT may be used to distinguish genera.

Original languageEnglish
Pages (from-to)170-172
Number of pages3
JournalInfrared Physics and Technology
Volume55
Issue number1
DOIs
StatePublished - Jan 2012

Bibliographical note

Funding Information:
We would like to thank Judy Glass for help with the experiments, and Mary Lou Cahal for assistance in preparing the figures. This study was supported in part by a fundamental research grant from the Institute of Research for Technology Development (IR4TD), University of Kentucky and with use of laboratory facilities at the Lexington Veterans Affairs Medical Center, Lexington, Kentucky.

Keywords

  • Bacterial quantification
  • Energy content
  • Infrared thermography
  • Real-time
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics

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