Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography

Ahmad A. Salaimeh; Jeffrey J. Campion; Belal Y. Gharaibeh; Martin E. Evans; Kozo Saito

doi:10.1016/j.infrared.2011.11.004

Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography

Ahmad A. Salaimeh
, Jeffrey J. Campion
, Belal Y. Gharaibeh
, Martin E. Evans
, Kozo Saito

Institute of Research for Technology Development (IR4TD)

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia coli, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to ∼4 × 10 ⁸ CFU/ml. The EC of S. aureus was ∼2-fold higher than that of E. coli at comparable cell concentrations suggesting that IRT may be used to distinguish genera.

Original language	English
Pages (from-to)	170-172
Number of pages	3
Journal	Infrared Physics and Technology
Volume	55
Issue number	1
DOIs	https://doi.org/10.1016/j.infrared.2011.11.004
State	Published - Jan 2012

Bibliographical note

Funding Information:
We would like to thank Judy Glass for help with the experiments, and Mary Lou Cahal for assistance in preparing the figures. This study was supported in part by a fundamental research grant from the Institute of Research for Technology Development (IR4TD), University of Kentucky and with use of laboratory facilities at the Lexington Veterans Affairs Medical Center, Lexington, Kentucky.

Funding

We would like to thank Judy Glass for help with the experiments, and Mary Lou Cahal for assistance in preparing the figures. This study was supported in part by a fundamental research grant from the Institute of Research for Technology Development (IR4TD), University of Kentucky and with use of laboratory facilities at the Lexington Veterans Affairs Medical Center, Lexington, Kentucky.

Funders	Funder number
University of Kentucky Institute of Research for Technology Development	IR4TD
University of Kentucky

Keywords

Bacterial quantification
Energy content
Infrared thermography
Real-time
Staphylococcus aureus

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Condensed Matter Physics

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10.1016/j.infrared.2011.11.004

Cite this

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title = "Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography",

abstract = "We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia coli, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to ∼4 {\texttimes} 10 8 CFU/ml. The EC of S. aureus was ∼2-fold higher than that of E. coli at comparable cell concentrations suggesting that IRT may be used to distinguish genera.",

keywords = "Bacterial quantification, Energy content, Infrared thermography, Real-time, Staphylococcus aureus",

author = "Salaimeh, \{Ahmad A.\} and Campion, \{Jeffrey J.\} and Gharaibeh, \{Belal Y.\} and Evans, \{Martin E.\} and Kozo Saito",

note = "Funding Information: We would like to thank Judy Glass for help with the experiments, and Mary Lou Cahal for assistance in preparing the figures. This study was supported in part by a fundamental research grant from the Institute of Research for Technology Development (IR4TD), University of Kentucky and with use of laboratory facilities at the Lexington Veterans Affairs Medical Center, Lexington, Kentucky.",

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language = "English",

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AU - Salaimeh, Ahmad A.

AU - Campion, Jeffrey J.

AU - Gharaibeh, Belal Y.

AU - Evans, Martin E.

AU - Saito, Kozo

N1 - Funding Information: We would like to thank Judy Glass for help with the experiments, and Mary Lou Cahal for assistance in preparing the figures. This study was supported in part by a fundamental research grant from the Institute of Research for Technology Development (IR4TD), University of Kentucky and with use of laboratory facilities at the Lexington Veterans Affairs Medical Center, Lexington, Kentucky.

PY - 2012/1

Y1 - 2012/1

N2 - We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia coli, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to ∼4 × 10 8 CFU/ml. The EC of S. aureus was ∼2-fold higher than that of E. coli at comparable cell concentrations suggesting that IRT may be used to distinguish genera.

AB - We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia coli, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to ∼4 × 10 8 CFU/ml. The EC of S. aureus was ∼2-fold higher than that of E. coli at comparable cell concentrations suggesting that IRT may be used to distinguish genera.

KW - Bacterial quantification

KW - Energy content

KW - Infrared thermography

KW - Real-time

KW - Staphylococcus aureus

UR - https://www.scopus.com/pages/publications/84655167891

UR - https://www.scopus.com/pages/publications/84655167891#tab=citedBy

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DO - 10.1016/j.infrared.2011.11.004

M3 - Article

AN - SCOPUS:84655167891

SN - 1350-4495

VL - 55

SP - 170

EP - 172

JO - Infrared Physics and Technology

JF - Infrared Physics and Technology

IS - 1

ER -

Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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Cite this