Recipient Cell Factors Influence Interbacterial Competition Mediated by Two Distinct Burkholderia dolosa Contact- Dependent Growth Inhibition Systems

Zaria K. Elery, A. Elizabeth Oates, Tanya Myers-Morales, Erin C. Garcia

Research output: Contribution to journalArticlepeer-review

Abstract

Contact-dependent growth inhibition (CDI) systems mediate interbacterial antagonism between Gram-negative bacteria by delivering the toxic portion of a large surface protein (termed BcpA in Burkholderia species) to the cytoplasm of neighboring bacteria. Translocation of the antibacterial polypeptide into recipient cells requires specific recipient outer and inner membrane proteins, but the identity of these factors outside several model organisms is unknown. To identify genes involved in CDI susceptibility in the Burkholderia cepacia complex member Burkholderia dolosa, a transposon mutagenesis selection approach was used to enrich for mutants resistant to BcpA-1 or BcpA-2. Subsequent analysis showed that candidate regulatory genes contributed modestly to recipient cell susceptibility to B. dolosa CDI. However, most candidate deletion mutants did not show the same phenotypes as the corresponding transposon mutants. Whole-genome resequencing revealed that these transposon mutants also contained unique mutations within a three gene locus (wabO, BDAG_01006, and BDAG_01005) encoding predicted lipopolysaccharide (LPS) biosynthesis enzymes. B. dolosa wabO, BDAG_01006, or BDAG_01005 mutants were resistant to CDI and produced LPS with altered core oligosaccharide and O-antigen. Although BcpA-1 and BcpA-2 are dissimilar and expected to utilize different outer membrane receptors, intoxication by both proteins was similarly impacted by LPS changes. Together, these findings suggest that alterations in cellular regulation may indirectly impact the efficiency of CDI-mediated competition and demonstrate that LPS is required for intoxication by two distinct B. dolosa BcpA proteins.

Original languageEnglish
JournalJournal of Bacteriology
Volume204
Issue number9
DOIs
StatePublished - Sep 2022

Bibliographical note

Funding Information:
This work was supported by a grant from the National Institutes of Health (R01AI150767 to E.C.G.) and start-up funding from the University of Kentucky.

Publisher Copyright:
© 2022 Elery et al.

Keywords

  • CDI
  • CdiA
  • LPS
  • interbacterial antagonism
  • lipopolysaccharide
  • two-partner secretion system
  • type V secretion

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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