Recognition of a DNA operator by the repressor of phage 434: A view at high resolution

Aneel K. Aggarwal, David W. Rodgers, Marie Drottar, Mark Ptashne, Stephen C. Harrison

Research output: Contribution to journalArticlepeer-review

478 Scopus citations

Abstract

The repressors of temperate bacteriophages such as 434 and lambda control transcription by binding to a set of DNA operator sites. The different affinity of repressor for each of these sites ensures efficient regulation. High-resolution x-ray crystallography was used to study the DNA-binding domain of phage 434 repressor in complex with a synthetic DNA operator. The structure shows recognition of the operator by direct interactions with base pairs in the ma or groove, combined with the sequence-dependent ability of DNA to adopt the required conformation on binding repressor. In particular, a network of three-centered bifurcated hydrogen bonds among base pairs in the operator helps explain why 434 repressor prefers certain sites over others. These bonds, which stabilize the conformation of the bound DNA, can form only with certain sequences.

Original languageEnglish
Pages (from-to)899-907
Number of pages9
JournalScience
Volume242
Issue number4880
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • General

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