Recombinant biglycan promotes bone morphogenetic protein-induced osteogenesis

P. A. Miguez, M. Terajima, H. Nagaoka, J. A.R. Ferreira, K. Braswell, C. C. Ko, M. Yamauchi

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The aim of this study was to determine the effects of glutathione-S- transferase-fused recombinant biglycan (GST-BGN) on craniofacial bone regeneration. We recently demonstrated a positive effect of tissue-derived BGN on bone morphogenetic protein 2 (BMP-2) function, which is exerted likely via the BGN core protein. Here, we investigated the effects of GST-BGN lacking any posttranslational modifications on BMP-2 function in vitro and in vivo. In the C2C12 cell culture system, BMP-2-induced Smad 1/5/8 phosphorylation and alkaline phosphatase activity were both enhanced by the addition of GST-BGN. For the in vivo effect, we employed a Sprague-Dawley rat mandible defect model utilizing 1 μg (optimal) or 0.1 μg (suboptimal) of BMP-2 combined with 0, 2, 4, or 8 μg of GST-BGN. At 2 weeks post-surgery, newly formed bone was evaluated by microcomputed tomography and histologic analyses. The results revealed that the greatest amounts of bone within the defect were formed in the groups of suboptimal BMP-2 combined with 4 or 8 μg of GST-BGN. Also, bone was well organized versus that formed by the optimal dose of BMP. These results indicate that recombinant BGN is an efficient substrate to promote low-dose BMP-induced osteogenesis.

Original languageEnglish
Pages (from-to)406-411
Number of pages6
JournalJournal of Dental Research
Volume93
Issue number4
DOIs
StatePublished - Apr 2014

Bibliographical note

Funding Information:
The study was supported in part by the National Institutes of Health (grants R21 DE020909 and AR060978 ).

Keywords

  • Smad 1/5/8
  • bone regeneration
  • critical-sized defect
  • growth factor
  • microcomputed tomography
  • proteoglycan

ASJC Scopus subject areas

  • General Dentistry

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