Recombinant E. coli prototype strains for in vivo glycorandomization

Gavin J. Williams, Jie Yang, Changsheng Zhang, Jon S. Thorson

Research output: Contribution to journalArticlepeer-review

59 Scopus citations


In vitro glycorandomization is a powerful strategy to alter the glycosylation patterns of natural products and small molecule therapeutics. Yet, such in vitro methods are often difficult to scale and can be costly given the requirement to provide various nucleotides and cofactors. Here, we report the construction of several recombinant E. coli prototype strains that allow the facile production of a range of small molecule glycosides. This strategy relies on the engineered promiscuity of three key enzymes, an anomeric kinase, a sugar-1-phosphate nucleotidyltransferase, and a glycosyltransferase, as well as the ability of diverse small molecules to freely enter E. coli. Subsequently, this work is the first demonstration of "in vivo glycorandomization" and offers vast combinatorial potential by simple fermentation.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalACS Chemical Biology
Issue number1
StatePublished - Jan 21 2011

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine


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