Recommendations for Design, Execution, and Reporting of Studies on Experimental Thoracic Aortopathy in Preclinical Models

doi:10.1161/ATVBAHA.124.320259

Recommendations for Design, Execution, and Reporting of Studies on Experimental Thoracic Aortopathy in Preclinical Models

Research output: Contribution to journal › Review article › peer-review

3 Scopus citations

Abstract

There is a recent dramatic increase in research on thoracic aortic diseases that includes aneurysms, dissections, and rupture. Experimental studies predominantly use mice in which aortopathy is induced by chemical interventions, genetic manipulations, or both. Many parameters should be deliberated in experimental design in concert with multiple considerations when providing dimensional data and characterization of aortic tissues. The purpose of this review is to provide recommendations on guidance in (1) the selection of a mouse model and experimental conditions for the study, (2) parameters for standardizing detection and measurements of aortic diseases, (3) meaningful interpretation of characteristics of diseased aortic tissue, and (4) reporting standards that include rigor and transparency.

Original language	English
Pages (from-to)	609-631
Number of pages	23
Journal	Arteriosclerosis, Thrombosis, and Vascular Biology
Volume	45
Issue number	5
DOIs	https://doi.org/10.1161/ATVBAHA.124.320259
State	Published - May 1 2025

Bibliographical note

Publisher Copyright:
© 2025 American Heart Association, Inc.

Funding

The authors are funded by the Leducq Foundation Network of Excellence (22CVD03). Other supports include National Institutes of Health grants R01HL109942 (D.M. Milewicz), R01HL158157 (Y.H. Shen and S.A. LeMaire), R01HL159988 (Y.H. Shen, K.B. Ghaghada, and S.A. LeMaire), U01HL142518, R01HL155105, R01HL169147 (J.D. Humphrey), and R35HL155649 (A. Daugherty). The authors were supported by the following American Heart Association awards: 19MERIT34480009 (D.M. Milewicz), 23MERIT1036341 (A. Daugherty), 24CDA1268148 (H. Sawada), and 23IPA1054637 (K.B. Ghaghada). Other grants include the British Heart Foundation (G101517 and RG96157 to Z. Mallat and T. Suzuki), National Institute for Health and Care Research, United Kingdom (T. Suzuki), and the Marfan Foundation (K.B. Ghaghada and Y. Li).

Funders	Funder number
National Institute for Health and Care Research
National Marfan Foundation
British Heart Foundation	RG96157, G101517
British Heart Foundation
American the American Heart Association	19MERIT34480009, 23MERIT1036341, 24CDA1268148, 23IPA1054637
American the American Heart Association
National Institutes of Health (NIH)	R01HL155105, R01HL158157, R01HL169147, U01HL142518, R01HL159988, R01HL109942, R35HL155649
National Institutes of Health (NIH)
Leducq Foundation Network of Excellence	22CVD03

Keywords

aneurysm
aorta
dissection
mice
research design

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine

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10.1161/ATVBAHA.124.320259

Cite this

@article{54f93ce846014246ae83ebd7fcc2f5c4,

title = "Recommendations for Design, Execution, and Reporting of Studies on Experimental Thoracic Aortopathy in Preclinical Models",

abstract = "There is a recent dramatic increase in research on thoracic aortic diseases that includes aneurysms, dissections, and rupture. Experimental studies predominantly use mice in which aortopathy is induced by chemical interventions, genetic manipulations, or both. Many parameters should be deliberated in experimental design in concert with multiple considerations when providing dimensional data and characterization of aortic tissues. The purpose of this review is to provide recommendations on guidance in (1) the selection of a mouse model and experimental conditions for the study, (2) parameters for standardizing detection and measurements of aortic diseases, (3) meaningful interpretation of characteristics of diseased aortic tissue, and (4) reporting standards that include rigor and transparency.",

keywords = "aneurysm, aorta, dissection, mice, research design",

author = "Alan Daugherty and Milewicz, \{Dianna M.\} and Dichek, \{David A.\} and Ghaghada, \{Ketan B.\} and Humphrey, \{Jay D.\} and Lemaire, \{Scott A.\} and Yanming Li and Ziad Mallat and Yvan Saeys and Hisashi Sawada and Shen, \{Ying H.\} and Toru Suzuki and Zhen Zhou",

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year = "2025",

month = may,

day = "1",

doi = "10.1161/ATVBAHA.124.320259",

language = "English",

volume = "45",

pages = "609--631",

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T1 - Recommendations for Design, Execution, and Reporting of Studies on Experimental Thoracic Aortopathy in Preclinical Models

AU - Daugherty, Alan

AU - Milewicz, Dianna M.

AU - Dichek, David A.

AU - Ghaghada, Ketan B.

AU - Humphrey, Jay D.

AU - Lemaire, Scott A.

AU - Li, Yanming

AU - Mallat, Ziad

AU - Saeys, Yvan

AU - Sawada, Hisashi

AU - Shen, Ying H.

AU - Suzuki, Toru

AU - Zhou, Zhen

PY - 2025/5/1

Y1 - 2025/5/1

N2 - There is a recent dramatic increase in research on thoracic aortic diseases that includes aneurysms, dissections, and rupture. Experimental studies predominantly use mice in which aortopathy is induced by chemical interventions, genetic manipulations, or both. Many parameters should be deliberated in experimental design in concert with multiple considerations when providing dimensional data and characterization of aortic tissues. The purpose of this review is to provide recommendations on guidance in (1) the selection of a mouse model and experimental conditions for the study, (2) parameters for standardizing detection and measurements of aortic diseases, (3) meaningful interpretation of characteristics of diseased aortic tissue, and (4) reporting standards that include rigor and transparency.

AB - There is a recent dramatic increase in research on thoracic aortic diseases that includes aneurysms, dissections, and rupture. Experimental studies predominantly use mice in which aortopathy is induced by chemical interventions, genetic manipulations, or both. Many parameters should be deliberated in experimental design in concert with multiple considerations when providing dimensional data and characterization of aortic tissues. The purpose of this review is to provide recommendations on guidance in (1) the selection of a mouse model and experimental conditions for the study, (2) parameters for standardizing detection and measurements of aortic diseases, (3) meaningful interpretation of characteristics of diseased aortic tissue, and (4) reporting standards that include rigor and transparency.

KW - aneurysm

KW - aorta

KW - dissection

KW - mice

KW - research design

UR - https://www.scopus.com/pages/publications/105000287016

UR - https://www.scopus.com/pages/publications/105000287016#tab=citedBy

U2 - 10.1161/ATVBAHA.124.320259

DO - 10.1161/ATVBAHA.124.320259

M3 - Review article

C2 - 40079138

AN - SCOPUS:105000287016

SN - 1079-5642

VL - 45

SP - 609

EP - 631

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

IS - 5

ER -

Recommendations for Design, Execution, and Reporting of Studies on Experimental Thoracic Aortopathy in Preclinical Models

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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