Recovery of primary transformants of soybean

W. A. Parrott, L. M. Hoffman, D. F. Hildebrand, E. G. Williams, G. B. Collins

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

Three transformants of soybean, Glycine max (L.) Merr., have been recovered among a total of 18 plants regenerated by somatic embryogenesis from immature cotyledon tissues after cocultivation with Agrobacterium strains carrying a 15 kD zein gene (pH5PZ3D). DNA from upper leaves hybridized to a synthetic RNA probe specific for the zein sequence at a level equivalent to at least one copy per haploid genome. Hybridization to a vir G/C probe, however, was negligible, indicating that sequestration of whole bacteria or even persistence of plasmids within the tissues could not account for the zein hybridization signals. Progeny of all plants were uniformly untransformed. Since most somatic embryos have a multicellular origin in the regeneration system used, it is believed that the primary transformants were chimeric. The results indicate that somatic embryogenesis may be adaptable to Agrobacterium-mediated transformation in soybean, but that greater numbers of mitotic cycles under selection before embryo initiation will be required if somatic embryogenesis is to be used efficiently for production of plants with transformed germ-line cells.

Original languageEnglish
Pages (from-to)615-617
Number of pages3
JournalPlant Cell Reports
Volume7
Issue number8
DOIs
StatePublished - Mar 1989

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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