Red cell Th activation: biochemical studies

Jay H. Herman, Wally Whiteheart, R. Sue Shirey, Robert J. Johnson, Thomas S. Kickler, Paul M. Ness

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Summary. The peanut agglutinin from Arachis hypogea is a lectin that reacts with red blood cells expressing the Th antigen. The Th antigen has been said to be qualitatively similar to the T antigen, a well‐defined antigen due to desialylation of glycophorin A and B that also reacts with the peanut agglutinin. We examined Th activated red blood cells from two patients with Fanconi's anaemia using 125I radio‐labelled peanut agglutinin as a probe in Western blotting of red blood cell membrane proteins. We also probed the surface of intact Th activated red blood cells for structures related to the T antigen using [3H]sialic acid and a purified sialyltransferase. Neither of these techniques found antigens on the Th activated red blood cells that were similar to the antigen found on T activated red blood cells. These results show that the Th antigen in Fanconi's anaemia is qualitatively different to the antigen found in T activation.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalBritish Journal of Haematology
Issue number2
StatePublished - Feb 1987

ASJC Scopus subject areas

  • Hematology


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