Terpenes constitute a distinct class of natural products that attract insects, defend against phytopathogenic microbes and combat human diseases. However, like most natural products, they are usually made by plants and microbes in small amounts and as complex mixtures. Chemical synthesis is often costly and inefficient, and may not yield enantiomerically pure terpenes, whereas large-scale microbial production requires expensive feedstocks. We engineered high-level terpene production in tobacco plants by diverting carbon flow from cytosolic or plastidic isopentenyl diphosphate through overexpression in either compartment of an avian farnesyl diphosphate synthase and an appropriate terpene synthase. Isotopic labeling studies suggest little, if any, metabolite exchange between these two subcellular compartments. The strategy increased synthesis of the sesquiterpenes patchoulol and amorpha-4,11-diene more than 1,000-fold, as well as the monoterpene limonene 10-30 fold, and seems equally suited to generating higher levels of other terpenes for research, industrial production or therapeutic applications.
|Number of pages||7|
|State||Published - Nov 2006|
Bibliographical noteFunding Information:
We thank Dale Poulter, University of Utah, for the avian farnesyl diphosphate synthase gene, Peter Brodelius, Kalmar University, for the amorpha-4,11-diene synthase gene and Randy Dinkins, University of Kentucky, for providing the Arabidopsis RUBISCO transit peptide sequence DNA. Special thanks to Nihar Nayak for his excellent advice concerning plant transformation, Scott Kinison for logistical and technical support, and Suphata Kaewpraparn for assistance with the insect bioassay experiment, all associated with the University of Kentucky. This work was supported by grants from Firmenich, Geneva (to J.C.) and from the US National Institutes of Health (GM 13956 to R.M.C).
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Molecular Medicine
- Biomedical Engineering