Redox couples of inducible nitric oxide synthase

Andrew K. Udit, Wendy Belliston-Bittner, Edith C. Glazer, Yen Hoang Le Nguyen, James M. Gillan, Michael G. Hill, Michael A. Marletta, David B. Goodin, Harry B. Gray

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

We report direct electrochemistry of the iNOS heme domain in a DDAB film on the surface of a basal plane graphite electrode. Cyclic voltammetry reveals FeIII/II and FeII/I couples at -191 and -1049 mV (vs Ag/AgCl). Imidazole and carbon monoxide in solution shift the FeIII/II potential by +20 and +62 mV, while the addition of dioxygen results in large catalytic waves at the onset of FeIII reduction. Voltammetry at higher scan rates (with pH variations) reveals that the FeIII/II cathodic peak can be resolved into two components, which are attributable to FeIII/II couples of five- and six-coordinate hemes. Digital simulation of our experimental data implicates water dissociation from the heme as a gating mechanism for ET in iNOS.

Original languageEnglish
Pages (from-to)11212-11213
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number32
DOIs
StatePublished - Aug 17 2005

ASJC Scopus subject areas

  • Catalysis
  • Chemistry (all)
  • Biochemistry
  • Colloid and Surface Chemistry

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