Redox proteomics in human biofluids: Sample preparation, separation and immunochemical tagging for analysis of protein oxidation

Fabio Di Domenico, Marzia Perluigi, D. Allan Butterfield

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Proteomics offers the simultaneous detection of a large number of proteins in a single experiment and can provide important information regarding crucial aspects of specific proteins, particularly post-translational modifications (PTMs). Investigations of oxidative PTMs are currently performed using focused redox proteomics techniques, which rely on gel electrophoresis separations of intact proteins with the final detection of oxidative PTMs being performed by mass spectrometry (MS) analysis. The application of this technique to human biofluids is being subject of increasing investigation and is expected to provide new insights on the oxidative status of the peripheral proteome in neurological diseases such as Alzheimer's disease, towards purposes of early diagnosis and prognosis. This chapter describes all the experimental steps to perform redox proteomics analysis of cerebrospinal fluid and plasma/serum samples.

Original languageEnglish
Title of host publicationSystems Biology of Alzheimer's Disease
Pages391-403
Number of pages13
ISBN (Electronic)9781493926275
DOIs
StatePublished - Aug 2 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015. All right reserved.

Keywords

  • Alzheimer's disease
  • Cerebrospinal fluid
  • Diagnosis
  • Neurological disorders
  • Oxidative stress
  • Plasma
  • Prognosis
  • Protein oxidation
  • Redox proteomics
  • Serum

ASJC Scopus subject areas

  • General Medicine
  • General Neuroscience

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