Reduced skeletal muscle satellite cell number alters muscle morphology after chronic stretch but allows limited serial sarcomere addition

Matthew C. Kinney, Sudarshan Dayanidhi, Peter B. Dykstra, John J. McCarthy, Charlotte A. Peterson, Richard L. Lieber

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Introduction: Muscles add sarcomeres in response to stretch, presumably to maintain optimal sarcomere length. Clinical evidence from patients with cerebral palsy, who have both decreased serial sarcomere number and reduced satellite cells (SCs), suggests a hypothesis that SCs may be involved in sarcomere addition. Methods: A transgenic Pax7-DTA mouse model underwent conditional SC depletion, and their soleii were then stretch-immobilized to assess the capacity for sarcomere addition. Muscle architecture, morphology, and extracellular matrix (ECM) changes were also evaluated. Results: Mice in the SC-reduced group achieved normal serial sarcomere addition in response to stretch. However, muscle fiber cross-sectional area was significantly smaller and was associated with hypertrophic ECM changes, consistent with fibrosis. Conclusions: While a reduced SC population does not hinder serial sarcomere addition, SCs play a role in muscle adaptation to chronic stretch that involves maintenance of both fiber cross-sectional area and ECM structure. Muscle Nerve 55: 384–392, 2017.

Original languageEnglish
Pages (from-to)384-392
Number of pages9
JournalMuscle and Nerve
Issue number3
StatePublished - Mar 1 2017

Bibliographical note

Funding Information:
None of the authors have any conflict of interest or financial disclosures related to this work. We appreciate the assistance of Dr. Simon Schenk with mouse treatment, Mary Esparza with muscle harvesting, and Dr. Gretchen Meyer with flow cytometry methods. The authors acknowledge NIH grants P30AR061303 and HD050837 and Department of Veterans Affairs grant A9028-R for support. This research was also aided by a grant from the Orthopaedic Research and Education Foundation, with Funding Provided by Depuy Synthes Joint Reconstruction.

Publisher Copyright:
© 2016 Wiley Periodicals, Inc.


  • cerebral palsy
  • chronic stretch
  • fibrosis
  • sarcomere addition
  • satellite cells
  • skeletal muscles

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)


Dive into the research topics of 'Reduced skeletal muscle satellite cell number alters muscle morphology after chronic stretch but allows limited serial sarcomere addition'. Together they form a unique fingerprint.

Cite this