RNA interference (RNAi) has become a widely used reverse genetic tool to study gene function in eukaryotic organisms and is being developed as a technology for insect pest management. The efficiency of RNAi varies among organisms. Insects from different orders also display differential efficiency of RNAi, ranging from highly efficient (coleopterans) to very low efficient (lepidopterans). We investigated the reasons for varying RNAi efficiency between lepidopteran and coleopteran cell lines and also between the Colorado potato beetle, Leptinotarsa decemlineata and tobacco budworm, Heliothis virescens. The dsRNA either injected or fed was degraded faster in H. virescens than in L. decemlineata. Both lepidopteran and coleopteran cell lines and tissues efficiently took up the dsRNA. Interestingly, the dsRNA administered to coleopteran cell lines and tissues was taken up and processed to siRNA whereas the dsRNA was taken up by lepidopteran cell lines and tissues but no siRNA was detected in the total RNA isolated from these cell lines and tissues. The data included in this paper showed that the degradation and intracellular transport of dsRNA are the major factors responsible for reduced RNAi efficiency in lepidopteran insects.
|Number of pages
|Published - Jul 2 2016
Bibliographical noteFunding Information:
This material is based upon work supported by the National Science Foundation I/UCRC, the Center for Arthropod Management Technologies under Grant No. IIP-1338775 and by industry partners. This is publication number 16-08-065 from the Kentucky Agricultural Experimental Station and published with the approval of the director. This work is supported by the National Institute of Food and Agriculture, USDA, HATCH under 2351177000. Special thanks to Dr. Cynthia Goodman for providing Lepd- SL1 and TcA cells and Dr. Seth DeBolt for the use of confocal microscope purchased by KY NSF EPSCoR via National Science Foundation award 1355438.
© 2016 Taylor & Francis Group, LLC.
- RNA interference
- double-stranded RNA
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology