Reduction midpoint potentials of bifurcating electron transfer flavoproteins

A. F. Miller, H. D. Duan, T. A. Varner, N. Mohamed Raseek

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

6 Scopus citations


Recently, a variety of enzymes have been found to accept electrons from NAD(P)H yet reduce lower-potential carriers such as ferredoxin and flavodoxin semiquinone, in apparent violation of thermodynamics. The reaction is favorable overall, however, because these enzymes couple the foregoing endergonic one-electron transfer to exergonic transfer of the other electron from each NAD(P)H, in a process called “flavin-based electron bifurcation.” The reduction midpoint potentials (E°s) of the multiple flavins in these enzymes are critical to their mechanisms. We describe methods we have found to be useful for measuring each of the E°s of each of the flavins in bifurcating electron transfer flavoproteins.

Original languageEnglish
Title of host publicationNew Approaches for Flavin Catalysis
EditorsBruce A. Palfey
Number of pages34
StatePublished - 2019

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Inc.


  • Electron bifurcation
  • Electron transfer flavoprotein
  • Flavin
  • Reduction midpoint potential
  • Spectroelectrochemistry

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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