Regional levels of phospholipase Cγ after fluid percussion brain injury in the rat

Heidi M. Carman; Harbhajan S. Dhillon; Deqing Zhang; James W. Geddes; Renuka M. Prasad

doi:10.1016/S0006-8993(98)00824-5

Regional levels of phospholipase Cγ after fluid percussion brain injury in the rat

Heidi M. Carman, Harbhajan S. Dhillon, Deqing Zhang, James W. Geddes, Renuka M. Prasad

Neuroscience

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Levels of PLCγ, a phospholipase C (PLC) isozyme, were significantly increased in the cytosol in the injured left cortex (LC) at 5, 30 and 120 min after brain injury. In the same site, although levels of membrane PLC γ did not alter at 5 and 30 min, they were found to be decreased at 2 h after brain injury. In general, the levels of both cytosolic and membrane PLC γ were unaltered in the contralateral right cortex (RC), ipsilateral left hippocampus (LH) and contralateral right hippocampus (RH) between 5 and 120 min after brain injury. These results suggest that, in addition to well- proposed excitatory neurotransmitter-receptor systems, increased levels of P LC γ may also contribute to alterations in PIP₂ signal transduction pathway, particularly in the greatest injury site (LC) after lateral EP brain injury.

Original language	English
Pages (from-to)	116-119
Number of pages	4
Journal	Brain Research
Volume	808
Issue number	1
DOIs	https://doi.org/10.1016/S0006-8993(98)00824-5
State	Published - Oct 12 1998

Bibliographical note

Funding Information:
This study was supported by funding from an NIH grant to Dr. M. Renuka Prasad (NS 34264). The authors thank Mrs. Sears and Morgan for the critical reading of the manuscript.

Keywords

Brain injury
Diacylglycerol
Fluid percussion
Inositol trisphosphate
Phosphatidylinositol bisphosphate
Phospholipase Cγ

ASJC Scopus subject areas

Neuroscience (all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(98)00824-5

Cite this

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title = "Regional levels of phospholipase Cγ after fluid percussion brain injury in the rat",

abstract = "Levels of PLCγ, a phospholipase C (PLC) isozyme, were significantly increased in the cytosol in the injured left cortex (LC) at 5, 30 and 120 min after brain injury. In the same site, although levels of membrane PLC γ did not alter at 5 and 30 min, they were found to be decreased at 2 h after brain injury. In general, the levels of both cytosolic and membrane PLC γ were unaltered in the contralateral right cortex (RC), ipsilateral left hippocampus (LH) and contralateral right hippocampus (RH) between 5 and 120 min after brain injury. These results suggest that, in addition to well- proposed excitatory neurotransmitter-receptor systems, increased levels of P LC γ may also contribute to alterations in PIP2 signal transduction pathway, particularly in the greatest injury site (LC) after lateral EP brain injury.",

keywords = "Brain injury, Diacylglycerol, Fluid percussion, Inositol trisphosphate, Phosphatidylinositol bisphosphate, Phospholipase Cγ",

author = "Carman, {Heidi M.} and Dhillon, {Harbhajan S.} and Deqing Zhang and Geddes, {James W.} and Prasad, {Renuka M.}",

note = "Funding Information: This study was supported by funding from an NIH grant to Dr. M. Renuka Prasad (NS 34264). The authors thank Mrs. Sears and Morgan for the critical reading of the manuscript.",

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AU - Carman, Heidi M.

AU - Dhillon, Harbhajan S.

AU - Zhang, Deqing

AU - Geddes, James W.

AU - Prasad, Renuka M.

N1 - Funding Information: This study was supported by funding from an NIH grant to Dr. M. Renuka Prasad (NS 34264). The authors thank Mrs. Sears and Morgan for the critical reading of the manuscript.

PY - 1998/10/12

Y1 - 1998/10/12

N2 - Levels of PLCγ, a phospholipase C (PLC) isozyme, were significantly increased in the cytosol in the injured left cortex (LC) at 5, 30 and 120 min after brain injury. In the same site, although levels of membrane PLC γ did not alter at 5 and 30 min, they were found to be decreased at 2 h after brain injury. In general, the levels of both cytosolic and membrane PLC γ were unaltered in the contralateral right cortex (RC), ipsilateral left hippocampus (LH) and contralateral right hippocampus (RH) between 5 and 120 min after brain injury. These results suggest that, in addition to well- proposed excitatory neurotransmitter-receptor systems, increased levels of P LC γ may also contribute to alterations in PIP2 signal transduction pathway, particularly in the greatest injury site (LC) after lateral EP brain injury.

AB - Levels of PLCγ, a phospholipase C (PLC) isozyme, were significantly increased in the cytosol in the injured left cortex (LC) at 5, 30 and 120 min after brain injury. In the same site, although levels of membrane PLC γ did not alter at 5 and 30 min, they were found to be decreased at 2 h after brain injury. In general, the levels of both cytosolic and membrane PLC γ were unaltered in the contralateral right cortex (RC), ipsilateral left hippocampus (LH) and contralateral right hippocampus (RH) between 5 and 120 min after brain injury. These results suggest that, in addition to well- proposed excitatory neurotransmitter-receptor systems, increased levels of P LC γ may also contribute to alterations in PIP2 signal transduction pathway, particularly in the greatest injury site (LC) after lateral EP brain injury.

KW - Brain injury

KW - Diacylglycerol

KW - Fluid percussion

KW - Inositol trisphosphate

KW - Phosphatidylinositol bisphosphate

KW - Phospholipase Cγ

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Regional levels of phospholipase Cγ after fluid percussion brain injury in the rat

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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