Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging

Alexandra E. Stanback; Lindsey R. Conroy; Lyndsay E.A. Young; Tara R. Hawkinson; Kia H. Markussen; Harrison A. Clarke; Derek B. Allison; Ramon C. Sun

doi:10.1016/j.xpro.2021.100304

Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging

Alexandra E. Stanback, Lindsey R. Conroy, Lyndsay E.A. Young, Tara R. Hawkinson, Kia H. Markussen, Harrison A. Clarke, Derek B. Allison, Ramon C. Sun

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is an innovative technique that enables in situ detection of analytes with spatial distribution. This workflow details a MALDI-MSI protocol for the spatial profiling of N-glycans and lipids from tissues following application of enzyme and MALDI matrix. For complete details on the use and execution of this protocol, please refer to Drake et al. (2018) and Andres et al. (2020).

Original language	English
Article number	100304
Journal	STAR Protocols
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2021.100304
State	Published - Mar 19 2021

Bibliographical note

Publisher Copyright:
© 2021 The Author(s)

Keywords

Mass spectrometry
Metabolism

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Neuroscience
General Immunology and Microbiology
General Medicine

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10.1016/j.xpro.2021.100304

Cite this

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title = "Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging",

abstract = "N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is an innovative technique that enables in situ detection of analytes with spatial distribution. This workflow details a MALDI-MSI protocol for the spatial profiling of N-glycans and lipids from tissues following application of enzyme and MALDI matrix. For complete details on the use and execution of this protocol, please refer to Drake et al. (2018) and Andres et al. (2020).",

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AU - Stanback, Alexandra E.

AU - Conroy, Lindsey R.

AU - Young, Lyndsay E.A.

AU - Hawkinson, Tara R.

AU - Markussen, Kia H.

AU - Clarke, Harrison A.

AU - Allison, Derek B.

AU - Sun, Ramon C.

PY - 2021/3/19

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N2 - N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is an innovative technique that enables in situ detection of analytes with spatial distribution. This workflow details a MALDI-MSI protocol for the spatial profiling of N-glycans and lipids from tissues following application of enzyme and MALDI matrix. For complete details on the use and execution of this protocol, please refer to Drake et al. (2018) and Andres et al. (2020).

AB - N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is an innovative technique that enables in situ detection of analytes with spatial distribution. This workflow details a MALDI-MSI protocol for the spatial profiling of N-glycans and lipids from tissues following application of enzyme and MALDI matrix. For complete details on the use and execution of this protocol, please refer to Drake et al. (2018) and Andres et al. (2020).

KW - Mass spectrometry

KW - Metabolism

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Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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