Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging

Alexandra E. Stanback, Lindsey R. Conroy, Lyndsay E.A. Young, Tara R. Hawkinson, Kia H. Markussen, Harrison A. Clarke, Derek B. Allison, Ramon C. Sun

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is an innovative technique that enables in situ detection of analytes with spatial distribution. This workflow details a MALDI-MSI protocol for the spatial profiling of N-glycans and lipids from tissues following application of enzyme and MALDI matrix. For complete details on the use and execution of this protocol, please refer to Drake et al. (2018) and Andres et al. (2020).

Original languageEnglish
Article number100304
JournalSTAR Protocols
Volume2
Issue number1
DOIs
StatePublished - Mar 19 2021

Bibliographical note

Publisher Copyright:
© 2021 The Author(s)

Keywords

  • Mass spectrometry
  • Metabolism

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience
  • General Immunology and Microbiology
  • General Medicine

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