Abstract
N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is an innovative technique that enables in situ detection of analytes with spatial distribution. This workflow details a MALDI-MSI protocol for the spatial profiling of N-glycans and lipids from tissues following application of enzyme and MALDI matrix. For complete details on the use and execution of this protocol, please refer to Drake et al. (2018) and Andres et al. (2020).
| Original language | English |
|---|---|
| Article number | 100304 |
| Journal | STAR Protocols |
| Volume | 2 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 19 2021 |
Bibliographical note
Publisher Copyright:© 2021 The Author(s)
Funding
This study was supported by NIH grant R01 AG066653, St. Baldrick's Career Development Award, Rally Foundation Independent Investigator Grant, and V-foundation Grant to R.C.S. and NIH Training Grant T32CA165990 to L.R.C. This research was also supported by funding from the University of Kentucky Markey Cancer Center and the NIH-funded Biospecimen Procurement & Translational Pathology Shared Resource Facility, as well as the Cancer Research Informatics Shared Resource Facility of the University of Kentucky Markey Cancer Center P30CA177558. R.C.S. conceptualized the study and designed the experimental workflow. L.E.A.Y. A.E.S. K.H.M. and T.R.H. optimized the protocol. K.H.M. T.R.H. and H.A.C. performed the experiments. R.C.S. L.R.C. D.B.A. K.H.M. and H.A.C. generated the figures. L.R.C. A.E.S. K.H.M. and R.C.S. wrote the manuscript. All authors read and approved the manuscript. The authors declare no competing interests. This study was supported by NIH grant R01 AG066653 , St. Baldrick’s Career Development Award, Rally Foundation Independent Investigator Grant, and V-foundation Grant to R.C.S., and NIH Training Grant T32CA165990 to L.R.C. This research was also supported by funding from the University of Kentucky Markey Cancer Center and the NIH -funded Biospecimen Procurement & Translational Pathology Shared Resource Facility, as well as the Cancer Research Informatics Shared Resource Facility of the University of Kentucky Markey Cancer Center P30CA177558 .
| Funders | Funder number |
|---|---|
| NIH-funded University of Kentucky Center for Cancer and Metabolism | |
| National Institutes of Health (NIH) | R01 AG066653 |
| National Childhood Cancer Registry – National Cancer Institute | P30CA177558 |
| Rally Foundation | T32CA165990 |
| University of Kentucky Markey Comprehensive Cancer Center |
Keywords
- Mass spectrometry
- Metabolism
ASJC Scopus subject areas
- General Neuroscience
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology