Regional quantification of myocardial mechanics in rat using 3D cine DENSE cardiovascular magnetic resonance

Xiaoyan Zhang, Zhan Qiu Liu, Dara Singh, Gregory J. Wehner, David K. Powell, Kenneth S. Campbell, Brandon K. Fornwalt, Jonathan F. Wenk

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Rat models have assumed an increasingly important role in cardiac research. However, a detailed profile of regional cardiac mechanics, such as strains and torsion, is lacking for rats. We hypothesized that healthy rat left ventricles (LVs) exhibit regional differences in cardiac mechanics, which are part of normal function. In this study, images of the LV were obtained with 3D cine displacement encoding with stimulated echoes (DENSE) cardiovascular magnetic resonance in 10 healthy rats. To evaluate regional cardiac mechanics, the LV was divided into basal, mid-ventricular, and apical regions. The myocardium at the mid-LV was further partitioned into four wall segments (i.e. septal, inferior, lateral, and anterior) and three transmural layers (i.e. sub-endocardium, mid-myocardium, and sub-epicardium). The six Lagrangian strain components (i.e. Err, Ecc, Ell, Ecl, Erl, and Ecr) were computed from the 3D displacement field and averaged within each region of interest. Torsion was quantified using the circumferential-longitudinal shear angle. While peak systolic Ecl differed between the mid-ventricle and apex, the other five components of peak systolic strain were similar across the base, mid-ventricle, and apex. In the mid-LV myocardium, Ecc decreased gradually from the sub-endocardial to the sub-epicardial layer. Ell demonstrated significant differences between the four wall segments, with the largest magnitude in the inferior segment. Err was uniform among the four wall segments. Ecl varied along the transmural direction and among wall segments, whereas Erl differed only among the wall segments. Erc was not associated with significant variations. Torsion also varied along the transmural direction and among wall segments. These results provide fundamental insights into the regional contractile function of healthy rat hearts, and form the foundation for future studies on regional changes induced by disease or treatments.

Original languageEnglish
Article numbere3733
JournalNMR in Biomedicine
Volume30
Issue number8
DOIs
StatePublished - Aug 2017

Bibliographical note

Funding Information:
The authors wish to thank Hua Wang and Amir Nikou for their help with CMR scans. This study was supported by an award from the American Heart Association (14BGIA18850020), a grant from the National Science Foundation (CMMI-1538754), and grants from the National Institutes of Health (S10RR029541, P20GM103527, UL1TR000117).

Publisher Copyright:
Copyright © 2017 John Wiley & Sons, Ltd.

Keywords

  • 3D cine DENSE CMR
  • rat
  • strain
  • torsion

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

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