Regulation by adrenocorticotropic hormone of the in vivo expression of scavenger receptor class B type I (SR-BI), a high density lipoprotein receptor, in steroidogenic cells of the murine adrenal gland

Attilio Rigotti, Elazer R. Edelman, Philip Seifer, Sohah N. Iqbal, Ronald B. DeMattos, Ryan E. Temel, Monty Krieger, David L. Williams

Research output: Contribution to journalArticlepeer-review

204 Scopus citations

Abstract

The class B, type I scavenger receptor, SR-BI, binds high density lipoprotein (HDD and can mediate selective uptake of HDL cholesteryl esters by cultured cells. The high levels of expression of SR-BI in steroidogenic tissues and the importance of selective uptake from HDL as a source of cholesterol for steroidogenesis raised the possibility that SR-BI may participate in cholesterol delivery to steroidogenic tissues in vivo. We have used immunoblotting and immunohistochemical methods to show that SR-BI is specifically expressed in a distinctive pattern on the surfaces of steroid-producing cells in the murine adrenal gland's cortex and that its expression in vivo is induced by adrenocorticotropic hormone and suppressed by glucocorticoids. Thus, expression of SR-BI protein is coordinately regulated with adrenal steroidogenesis. These data provide strong support for the hypothesis that SR-BI is a physiologically relevant HDL receptor that provides substrate cholesterol for steroid hormone synthesis.

Original languageEnglish
Pages (from-to)33545-33549
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number52
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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