TY - JOUR
T1 - Regulation of a novel gene encoding a lysyl oxidase-related protein in cellular adhesion and senescence
AU - Saito, Hiroshi
AU - Papaconstantinou, John
AU - Sato, Hiroyuki
AU - Goldstein, Samuel
PY - 1997/3/28
Y1 - 1997/3/28
N2 - We report here a novel cDNA clone with a predicted protein sequence similar to lysyl oxidase. This full-length cDNA clone of 3432 base pairs (WS9-14) was isolated from human fibroblasts on the basis of its overexpression in senescent cells. It encodes an 87-kDa polypeptide, whose protein is a member of the scavenger receptor cysteine-rich family, because it contains four scavenger receptor cysteine-rich domains that are found in several secreted or cell surface proteins. The WS9-14 protein has a 48% identity with both lysyl oxidase and lysyl oxidase-like protein at a region corresponding to exons 2-6, implying the existence of a lysyl oxidase gene family. The pattern of WS9-14 gene expression by fibroblasts parallels pro- collagen I-α1 expression. Its mRNA level is induced by transforming growth factor β-1 and indomethacin and inhibited by phorbol ester and retinoic acid. WS9-14 is abundantly expressed in all tumor cell lines examined that attach to culture dishes but not in cell lines that grow in suspension and is also up-regulated in senescent fibroblasts. These results suggest that WS9- 14 gene encodes an extracellular protein that may be specifically involved in cell adhesion and senescence.
AB - We report here a novel cDNA clone with a predicted protein sequence similar to lysyl oxidase. This full-length cDNA clone of 3432 base pairs (WS9-14) was isolated from human fibroblasts on the basis of its overexpression in senescent cells. It encodes an 87-kDa polypeptide, whose protein is a member of the scavenger receptor cysteine-rich family, because it contains four scavenger receptor cysteine-rich domains that are found in several secreted or cell surface proteins. The WS9-14 protein has a 48% identity with both lysyl oxidase and lysyl oxidase-like protein at a region corresponding to exons 2-6, implying the existence of a lysyl oxidase gene family. The pattern of WS9-14 gene expression by fibroblasts parallels pro- collagen I-α1 expression. Its mRNA level is induced by transforming growth factor β-1 and indomethacin and inhibited by phorbol ester and retinoic acid. WS9-14 is abundantly expressed in all tumor cell lines examined that attach to culture dishes but not in cell lines that grow in suspension and is also up-regulated in senescent fibroblasts. These results suggest that WS9- 14 gene encodes an extracellular protein that may be specifically involved in cell adhesion and senescence.
UR - http://www.scopus.com/inward/record.url?scp=0030888680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030888680&partnerID=8YFLogxK
U2 - 10.1074/jbc.272.13.8157
DO - 10.1074/jbc.272.13.8157
M3 - Article
C2 - 9079631
AN - SCOPUS:0030888680
SN - 0021-9258
VL - 272
SP - 8157
EP - 8160
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 13
ER -