Regulation of herpes simplex virus-specific cell-mediated immunity by a specific suppressor factor

D. W. Horohov, J. H. Wyckoff, R. N. Moore, B. T. Rouse

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Our study was designed to investigate the nature of an antigen-specific suppressor factor generated by antigen-stimulated herpes simplex virus (HSV)-immune splenocytes. Factor SF-200, a 90,000- to 100,000-dalton fraction obtained after Sephacryl gel filtration, suppressed the generation of HSV-specific cytotoxic T-lymphocyte and lymphoproliferative responses. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis of SF-200 indicated that it contained an I-J+, anti-idiotypic protein. It was possible to adsorb the suppressor activity of SF-200 to an anti-I-J immunoaffinity column. The suppressor activity could be eluted from the immunoaffinity column with a low-pH buffer. The acid-eluted material was determined to be both I-J+ and reactive with anti-HSV antiserum by Western blot analysis. Both SF-200 and the I-J+ suppressor activity suppressed only HSV-specific cell-mediated immunity responses. However, it was possible to generate nonspecific suppressor activity by incubating the I-J+ suppressor factor with Lyt 1+ splenocytes from HSV-immune mice. The implication of these results with respect to the model for a suppressor cell circuit regulating HSV-specific cell-mediated immunity responses is discussed.

Original languageEnglish
Pages (from-to)331-338
Number of pages8
JournalJournal of Virology
Volume58
Issue number2
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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