Regulation of induced pluripotent stem (iPS) cell induction by Wnt/β-catenin signaling

Peilin Zhang; Wen Hsuan Chang; Brendan Fong; Fan Gao; Chunming Liu; Denise Al Alam; Saverio Bellusci; Wange Lu

doi:10.1074/jbc.M113.542845

Regulation of induced pluripotent stem (iPS) cell induction by Wnt/β-catenin signaling

Peilin Zhang, Wen Hsuan Chang, Brendan Fong, Fan Gao, Chunming Liu, Denise Al Alam, Saverio Bellusci, Wange Lu

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Wnt signaling has been implicated in promoting somatic cell reprogramming. However, its molecular mechanisms remain unknown. Here we report that Wnt/β-catenin enhances iPSCs induction at the early stage of reprogramming. The augmented reprogramming induced by β-catenin is not due to increased total cell population or activation of c-Myc. In addition, β-catenin interacts with reprogramming factors Klf4, Oct4, and Sox2, further enhancing expression of pluripotency circuitry genes. These studies reveal novel mechanisms underlying the regulation of reprogramming somatic cells to pluripotency by Wnt/β-catenin signaling.

Original language	English
Pages (from-to)	9221-9232
Number of pages	12
Journal	Journal of Biological Chemistry
Volume	289
Issue number	13
DOIs	https://doi.org/10.1074/jbc.M113.542845
State	Published - Mar 30 2014

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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title = "Regulation of induced pluripotent stem (iPS) cell induction by Wnt/β-catenin signaling",

abstract = "Wnt signaling has been implicated in promoting somatic cell reprogramming. However, its molecular mechanisms remain unknown. Here we report that Wnt/β-catenin enhances iPSCs induction at the early stage of reprogramming. The augmented reprogramming induced by β-catenin is not due to increased total cell population or activation of c-Myc. In addition, β-catenin interacts with reprogramming factors Klf4, Oct4, and Sox2, further enhancing expression of pluripotency circuitry genes. These studies reveal novel mechanisms underlying the regulation of reprogramming somatic cells to pluripotency by Wnt/β-catenin signaling.",

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AU - Alam, Denise Al

AU - Bellusci, Saverio

AU - Lu, Wange

PY - 2014/3/30

Y1 - 2014/3/30

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AB - Wnt signaling has been implicated in promoting somatic cell reprogramming. However, its molecular mechanisms remain unknown. Here we report that Wnt/β-catenin enhances iPSCs induction at the early stage of reprogramming. The augmented reprogramming induced by β-catenin is not due to increased total cell population or activation of c-Myc. In addition, β-catenin interacts with reprogramming factors Klf4, Oct4, and Sox2, further enhancing expression of pluripotency circuitry genes. These studies reveal novel mechanisms underlying the regulation of reprogramming somatic cells to pluripotency by Wnt/β-catenin signaling.

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Regulation of induced pluripotent stem (iPS) cell induction by Wnt/β-catenin signaling

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