TY - JOUR
T1 - Regulation of Par-4 by Oncogenic Ras
AU - Murthi Vasudevan, Krishna
AU - Ranganathan, Padhma
AU - Rangnekar, Vivek M.
PY - 2006
Y1 - 2006
N2 - Oncogenic Ras causes down-regulation of the proapoptotic tumor suppressor gene Par-4. Replenishment of the basal levels of Par-4 results in inhibition of Ras-inducible cellular transformation. Moreover, overexpression of Par-4 (twofold to fourfold over basal levels) results in apoptosis of cells expressing oncogenic Ras. Par-4 does not, on its own, induce apoptosis in immortalized or nontransformed cells. This chapter describes the key methods used for analysis of Par-4 down-regulation by oncogenic Ras, which can be extended to study most genes whose down-regulation by oncogenic Ras is critical for oncogenic transformation and cell survival.
AB - Oncogenic Ras causes down-regulation of the proapoptotic tumor suppressor gene Par-4. Replenishment of the basal levels of Par-4 results in inhibition of Ras-inducible cellular transformation. Moreover, overexpression of Par-4 (twofold to fourfold over basal levels) results in apoptosis of cells expressing oncogenic Ras. Par-4 does not, on its own, induce apoptosis in immortalized or nontransformed cells. This chapter describes the key methods used for analysis of Par-4 down-regulation by oncogenic Ras, which can be extended to study most genes whose down-regulation by oncogenic Ras is critical for oncogenic transformation and cell survival.
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U2 - 10.1016/S0076-6879(05)07035-7
DO - 10.1016/S0076-6879(05)07035-7
M3 - Review article
C2 - 16757343
AN - SCOPUS:33744552178
SN - 0076-6879
VL - 407
SP - 422
EP - 442
JO - Methods in Enzymology
JF - Methods in Enzymology
ER -