Regulation of Par-4 by Oncogenic Ras

Krishna Murthi Vasudevan, Padhma Ranganathan, Vivek M. Rangnekar

Research output: Contribution to journalReview articlepeer-review

2 Scopus citations

Abstract

Oncogenic Ras causes down-regulation of the proapoptotic tumor suppressor gene Par-4. Replenishment of the basal levels of Par-4 results in inhibition of Ras-inducible cellular transformation. Moreover, overexpression of Par-4 (twofold to fourfold over basal levels) results in apoptosis of cells expressing oncogenic Ras. Par-4 does not, on its own, induce apoptosis in immortalized or nontransformed cells. This chapter describes the key methods used for analysis of Par-4 down-regulation by oncogenic Ras, which can be extended to study most genes whose down-regulation by oncogenic Ras is critical for oncogenic transformation and cell survival.

Original languageEnglish
Pages (from-to)422-442
Number of pages21
JournalMethods in Enzymology
Volume407
DOIs
StatePublished - 2006

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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