Regulation of SIRT6 protein levels by nutrient availability

Yariv Kanfi; Ronnie Shalman; Victoria Peshti; Shmuel N. Pilosof; Yosi M. Gozlan; Kevin J. Pearson; Batya Lerrer; Danesh Moazed; Jean Christophe Marine; Rafael de Cabo; Haim Y. Cohen

doi:10.1016/j.febslet.2008.01.019

Regulation of SIRT6 protein levels by nutrient availability

Yariv Kanfi, Ronnie Shalman, Victoria Peshti, Shmuel N. Pilosof, Yosi M. Gozlan, Kevin J. Pearson, Batya Lerrer, Danesh Moazed, Jean Christophe Marine, Rafael de Cabo, Haim Y. Cohen

Research output: Contribution to journal › Article › peer-review

154 Scopus citations

Abstract

Sirtuins have been shown to regulate life-span in response to nutritional availability. We show here that levels of the mammalian sirtuin, SIRT6, increased upon nutrient deprivation in cultured cells, in mice after fasting, and in rats fed a calorie-restricted diet. The increase in SIRT6 levels is due to stabilization of SIRT6 protein, and not via an increase in SIRT6 transcription. In addition, p53 positively regulates SIRT6 protein levels under standard growth conditions but has no role in the nutrient-dependent regulation of SIRT6. These observations imply that at least two sirtuins are involved in regulation of life-span by nutrient availability.

Original language	English
Pages (from-to)	543-548
Number of pages	6
Journal	FEBS Letters
Volume	582
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2008.01.019
State	Published - Mar 5 2008

Bibliographical note

Funding Information:
We thank Doron Ginsberg (Bar-Ilan University, Israel) and members of the Cohen lab for helpful comments on the manuscript; Fred Alt (HMS) for the SIRT6 −/− mice; Moshe Oren (Weizmann Institute, Israel) for the p53 −/− mice; and Izumi Horikawa (NIH) for pcDNA-DEST40-SIRT6. This study was supported by grants from the Israeli Academy of Sciences, German–Israeli Foundation, Binational US–Israel Foundation, Israel Cancer Association, Koret Foundation, and the Israel Cancer Research Foundation. H.C. is funded by the Alon Foundation.

Keywords

Calorie restriction
Nutrient availability
SIRT6
Sirtuin

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/j.febslet.2008.01.019

Cite this

@article{3ac9e1aa236b4ff8849e19b8e4c241fd,

title = "Regulation of SIRT6 protein levels by nutrient availability",

abstract = "Sirtuins have been shown to regulate life-span in response to nutritional availability. We show here that levels of the mammalian sirtuin, SIRT6, increased upon nutrient deprivation in cultured cells, in mice after fasting, and in rats fed a calorie-restricted diet. The increase in SIRT6 levels is due to stabilization of SIRT6 protein, and not via an increase in SIRT6 transcription. In addition, p53 positively regulates SIRT6 protein levels under standard growth conditions but has no role in the nutrient-dependent regulation of SIRT6. These observations imply that at least two sirtuins are involved in regulation of life-span by nutrient availability.",

keywords = "Calorie restriction, Nutrient availability, SIRT6, Sirtuin",

author = "Yariv Kanfi and Ronnie Shalman and Victoria Peshti and Pilosof, {Shmuel N.} and Gozlan, {Yosi M.} and Pearson, {Kevin J.} and Batya Lerrer and Danesh Moazed and Marine, {Jean Christophe} and {de Cabo}, Rafael and Cohen, {Haim Y.}",

note = "Funding Information: We thank Doron Ginsberg (Bar-Ilan University, Israel) and members of the Cohen lab for helpful comments on the manuscript; Fred Alt (HMS) for the SIRT6 −/− mice; Moshe Oren (Weizmann Institute, Israel) for the p53 −/− mice; and Izumi Horikawa (NIH) for pcDNA-DEST40-SIRT6. This study was supported by grants from the Israeli Academy of Sciences, German–Israeli Foundation, Binational US–Israel Foundation, Israel Cancer Association, Koret Foundation, and the Israel Cancer Research Foundation. H.C. is funded by the Alon Foundation. ",

year = "2008",

month = mar,

day = "5",

doi = "10.1016/j.febslet.2008.01.019",

language = "English",

volume = "582",

pages = "543--548",

number = "5",

}

TY - JOUR

T1 - Regulation of SIRT6 protein levels by nutrient availability

AU - Kanfi, Yariv

AU - Shalman, Ronnie

AU - Peshti, Victoria

AU - Pilosof, Shmuel N.

AU - Gozlan, Yosi M.

AU - Pearson, Kevin J.

AU - Lerrer, Batya

AU - Moazed, Danesh

AU - Marine, Jean Christophe

AU - de Cabo, Rafael

AU - Cohen, Haim Y.

N1 - Funding Information: We thank Doron Ginsberg (Bar-Ilan University, Israel) and members of the Cohen lab for helpful comments on the manuscript; Fred Alt (HMS) for the SIRT6 −/− mice; Moshe Oren (Weizmann Institute, Israel) for the p53 −/− mice; and Izumi Horikawa (NIH) for pcDNA-DEST40-SIRT6. This study was supported by grants from the Israeli Academy of Sciences, German–Israeli Foundation, Binational US–Israel Foundation, Israel Cancer Association, Koret Foundation, and the Israel Cancer Research Foundation. H.C. is funded by the Alon Foundation.

PY - 2008/3/5

Y1 - 2008/3/5

N2 - Sirtuins have been shown to regulate life-span in response to nutritional availability. We show here that levels of the mammalian sirtuin, SIRT6, increased upon nutrient deprivation in cultured cells, in mice after fasting, and in rats fed a calorie-restricted diet. The increase in SIRT6 levels is due to stabilization of SIRT6 protein, and not via an increase in SIRT6 transcription. In addition, p53 positively regulates SIRT6 protein levels under standard growth conditions but has no role in the nutrient-dependent regulation of SIRT6. These observations imply that at least two sirtuins are involved in regulation of life-span by nutrient availability.

AB - Sirtuins have been shown to regulate life-span in response to nutritional availability. We show here that levels of the mammalian sirtuin, SIRT6, increased upon nutrient deprivation in cultured cells, in mice after fasting, and in rats fed a calorie-restricted diet. The increase in SIRT6 levels is due to stabilization of SIRT6 protein, and not via an increase in SIRT6 transcription. In addition, p53 positively regulates SIRT6 protein levels under standard growth conditions but has no role in the nutrient-dependent regulation of SIRT6. These observations imply that at least two sirtuins are involved in regulation of life-span by nutrient availability.

KW - Calorie restriction

KW - Nutrient availability

KW - SIRT6

KW - Sirtuin

UR - http://www.scopus.com/inward/record.url?scp=39649100868&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39649100868&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2008.01.019

DO - 10.1016/j.febslet.2008.01.019

M3 - Article

C2 - 18242175

AN - SCOPUS:39649100868

SN - 0014-5793

VL - 582

SP - 543

EP - 548

JO - FEBS Letters

JF - FEBS Letters

IS - 5

ER -

Regulation of SIRT6 protein levels by nutrient availability

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this